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Osteoarticular vascular corrosion casting using industrial polyurethane for the 3D representation of the vascular tree on human knee
Annals of Anatomy ( IF 2.0 ) Pub Date : 2021-08-12 , DOI: 10.1016/j.aanat.2021.151816
Fatos Ramadani 1 , Daniel Petek 1 , Moritz Tannast 1 , Luis Filgueira 2
Affiliation  

Vascular casting is a widely used method for the representation of body vascularization. Many different injection materials have been described throughout the time to enhance the arterial vascular supply within a specifically defined anatomical location. The use of industrial polyurethane has been recently evaluated and applied to animal and human anatomy. The aim of this study was to confirm the safe and reliable use of industrial polyurethane in knee specimen in order to obtain a three-dimensional vascular tree of the distal femur. 10 fresh-frozen knees (mid-thigh to mid tibia) were used to assess the vascularity around the femoral condyles. Industrial polyurethane foam (Soudal™ foam) was diluted with acetone in order to obtain a runny fluid, easy to inject. After injection, the knees were bathed in a 10% NaOH solution, heated at 30°. The corrosion process took from 20 to 24 h and allowed all the soft tissue surrounding the knee to be subsided, leaving only the bone with polyurethane vascular architecture. After soft tissue corrosion, the vascular network around the knees was easily identified underlying the relation of the vessels to the bone. Even small arterioles (diameter< 1 mm) were distinguished with a good resistance to breakage. Corrosion casting remains an easy and reliable alternative to dissection for the understanding of tissue perfusion as the handling of the polyurethane is easy and has low costs. The described author’s method can be used osteo-articular specimen as well as in other organs. The protocol of injection and corrosion needs however to be adapted to the different specimen and anatomical location. Polyurethane associated to acetone can safely be used as injection material in order to demonstrate the vascularity of a specimen and remains easy to use.



中文翻译:

使用工业聚氨酯的骨关节血管腐蚀铸件用于人体膝关节血管树的 3D 表示

血管铸型是一种广泛使用的表示身体血管化的方法。许多不同的注射材料一直被描述以增强特定解剖位置内的动脉血管供应。最近对工业聚氨酯的使用进行了评估,并将其应用于动物和人体解剖学。本研究的目的是确认工业聚氨酯在膝关节标本中的安全可靠使用,以获得股骨远端的三维血管树。10 个新鲜冷冻的膝盖(大腿中部至胫骨中部)用于评估股骨髁周围的血管分布。工业聚氨酯泡沫(Soudal™ 泡沫)用丙酮稀释以获得易于注射的流动流体。注射后,将膝盖浸泡在 10% NaOH 溶液中,加热至 30°。 h 并允许膝关节周围的所有软组织消退,仅留下具有聚氨酯血管结构的骨骼。软组织腐蚀后,膝盖周围的血管网络很容易被识别,这是血管与骨骼关系的基础。即使是小动脉(直径<  1 mm) 具有良好的抗破损性。腐蚀铸造仍然是解剖的一种简单可靠的替代方法,用于了解组织灌注,因为聚氨酯的处理简单且成本低。所描述的作者的方法可用于骨关节标本以及其他器官。然而,注射和腐蚀的协议需要适应不同的标本和解剖位置。与丙酮相关的聚氨酯可以安全地用作注射材料,以证明样本的血管分布并保持易于使用。

更新日期:2021-08-27
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