ABSTRACT

Leaf morphogenesis in Vitis vinifera is closely associated with the biosynthesis of various metabolites, starch, and growth-related molecules. In the present study, Illumina RNA deep sequencing-based on transcriptome analysis was deployed to understand leaf morphogenesis in Vitis vinifera. The KEGG pathway analysis revealed the transcripts involved in the biosynthesis of major metabolites leading to leaf growth. The highest number of DEGs were down-regulated at young leave-vs-mature leaves stages (3,501) followed by large leaves-vs-mature leaves (2,364); however, the minimum number of up-regulated transcripts were recorded at young leaves-vs-immature leaves (195). The gene regulation in the starch biosynthetic pathway, young leaves-vs-mature leaves showed the highest number of transcripts (24 up-regulated and 60 down-regulated). For ABC Transporters, 224 transcripts were expressed, among which the maximum number of transcripts (59) were recorded in large leaves-vs-mature leaves. Amino and nucleotide sugar metabolism showed 112 transcripts (22 up-regulated and 90 down-regulated), in which immature leaves-vs-mature leaves expressed 30 DEGs. In addition, Flavonoid pathway showed the highest number of transcripts i.e. 903 were expressed among the studied pathways. The findings of this study will also help to a better understanding of the underlying process during grape leaf morphogenesis and to unravel the mechanism.

摘要

葡萄的叶片形态发生与各种代谢物、淀粉和生长相关分子的生物合成密切相关。在本研究中，基于转录组分析的 Illumina RNA 深度测序被用于了解葡萄的叶片形态发生. KEGG 通路分析揭示了参与导致叶片生长的主要代谢物生物合成的转录物。在幼叶与成熟叶阶段（3,501 个）下调的 DEG 数量最多，其次是大叶与成熟叶阶段（2,364 个）；然而，在幼叶与未成熟叶中记录的上调转录物的最小数量（195）。淀粉生物合成途径中的基因调控，幼叶与成熟叶的转录本数量最多（24 个上调和 60 个下调）。对于 ABC 转运蛋白，表达了 224 个转录本，其中最大数量的转录本 (59) 记录在大叶与成熟叶中。氨基和核苷酸糖代谢显示 112 个转录物（22 个上调和 90 个下调），其中未成熟叶对成熟叶表达 30°。此外，类黄酮途径显示出最多的转录本，即在研究的途径中表达了 903 个。这项研究的结果也将有助于更好地了解葡萄叶形态发生的潜在过程并揭示其机制。