Confocal Raman spectroscopy (CRS) has shown potential in non-invasive skin analysis. However, current CRS systems have various limitations including a narrow detection band, large size, non-flexibility, slowness, and complexity, which hinder their clinical applications. Herein, we developed a portable ultrawideband CRS system with a fiber-based handheld probe to acquire the Raman spectra in both fingerprint and high wavenumber regions in a fast and quasi-simultaneous way. Dual-wavelength excitation with a dual-passband laser cleaning filter and high-speed fiber array multiplexer was adopted instead of a specialized grating and detector to achieve instant switching between the detection regions and improve system robustness. Preliminary in vivo results demonstrated its depth profiling capability in an ultrawide detection range for stratum corneum thickness, natural moisturizing factor, and water content quantification, indicating its great potential in a wide range of clinical and cosmeceutical applications.

中文翻译：

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用于皮肤研究的带有手持式探头的便携式超宽带共焦拉曼光谱系统

共聚焦拉曼光谱 (CRS) 已在非侵入性皮肤分析中显示出潜力。然而，目前的CRS系统存在检测范围窄、体积大、不灵活、速度慢、复杂等诸多局限性，阻碍了其临床应用。在此，我们开发了一种带有基于光纤的手持式探头的便携式超宽带 CRS 系统，以快速且准同时的方式获取指纹和高波数区域的拉曼光谱。采用双通带激光清洗滤光片和高速光纤阵列多路复用器的双波长激发代替专门的光栅和检测器，实现检测区域的即时切换，提高系统鲁棒性。体内初步 结果表明其在角质层厚度、天然保湿因子和含水量量化的超宽检测范围内具有深度分析能力，表明其在广泛的临床和药妆应用中具有巨大潜力。