Species-specific diagnosis still represents a challenge in leishmaniasis management, particularly in regions with multiple endemic species. In Brazil, seven species have been recognized as etiological agents of cutaneous leishmaniasis. The disease comprises complex clinical presentation patterns, classified as localized, diffuse, disseminated and mucocutaneous leishmaniasis. In this study, we characterized the full nucleotide sequence of a region comprising the ribosomal DNA internal transcribed spacers 1 and 2 and 5.8 S gene of reference strains of Leishmania (Viannia) species reported as causative agents of human leishmaniasis in Brazil. The analysis of the nucleotide sequence of this region was able to discriminate species in the Leishmania (Viannia) subgenus and to determine intra- and interspecies phylogenetic relationships.

物种特异性诊断仍然是利什曼病管理的一个挑战，特别是在有多种地方性物种的地区。在巴西，有七种物种已被确认为皮肤利什曼病的病原体。该疾病具有复杂的临床表现模式，分为局限性利什曼病、弥漫性利什曼病、播散性利什曼病和皮肤粘膜利什曼病。在这项研究中，我们对被报道为巴西人类利什曼病病原体的利什曼原虫（Viannia）参考菌株的核糖体 DNA 内转录间隔区 1 和 2 以及 5.8 S 基因组成的区域的完整核苷酸序列进行了表征。对该区域的核苷酸序列的分析能够区分利什曼原虫（ Viannia ）亚属的物种并确定种内和种间的系统发育关系。