Species-specific diagnosis still represents a challenge in leishmaniasis management, particularly in regions with multiple endemic species. In Brazil, seven species have been recognized as etiological agents of cutaneous leishmaniasis. The disease comprises complex clinical presentation patterns, classified as localized, diffuse, disseminated and mucocutaneous leishmaniasis. In this study, we characterized the full nucleotide sequence of a region comprising the ribosomal DNA internal transcribed spacers 1 and 2 and 5.8 S gene of reference strains of Leishmania (Viannia) species reported as causative agents of human leishmaniasis in Brazil. The analysis of the nucleotide sequence of this region was able to discriminate species in the Leishmania (Viannia) subgenus and to determine intra- and interspecies phylogenetic relationships.

物种特异性诊断仍然是利什曼病管理的一个挑战，特别是在有多种地方性物种的地区。在巴西，有七种物种已被确认为皮肤利什曼病的病原体。该疾病包括复杂的临床表现模式，分为局限性、弥漫性、播散性和皮肤粘膜利什曼病。在这项研究中，我们表征了一个区域的完整核苷酸序列，该区域包含核糖体 DNA 内部转录间隔区 1 和 2 以及利什曼原虫 (Viannia)物种参考菌株的 5.8 S 基因，这些参考菌株被报告为巴西人类利什曼病的病原体。对该区域核苷酸序列的分析能够区分利什曼原虫（Viannia) 亚属并确定种内和种间的系统发育关系。