As DNA metabarcoding has become an emerging tool for surveying biodiversity, including its application in legally binding assessments, reliable and efficient barcodes are requested, especially for the highly diverse group of zooplankton. This study focuses on comparing the efficiency of two mitochondrial COI barcodes based on the internal primers mlCOIintF and mlCOIintR utilizing mesozooplankton samples collected in a Mediterranean lagoon. Our results indicate that after a slight adjustment, the mlCOIintR primer performs in combination with jdgLCO1490 (herein) very comparably to the much more widely used primer system mlCOIintF/jgHCO2198+dgHCO2198, in terms of level of taxonomic resolution, species detection and their relative abundance in terms of numbers of reads. As for some groups, like Ctenophora, this barcode is not suitable; a combination of them may be the best option to rely on the Folmer region in its entirety without the risk of losing information for a limited primer match.

由于 DNA 元条形码已成为调查生物多样性的新兴工具，包括其在具有法律约束力的评估中的应用，因此需要可靠和高效的条形码，尤其是对于高度多样化的浮游动物群体。本研究的重点是比较基于内部引物 mlCOIintF 和 mlCOIintR 的两个线粒体 COI 条形码的效率，利用在地中海泻湖中收集的中型浮游动物样本。我们的结果表明，经过轻微调整后，mlCOIintR 引物与 jdgLCO1490（本文中）的结合与使用更广泛的引物系统 mlCOIintF/jgHCO2198+dgHCO2198 在分类分辨率、物种检测及其相对丰度方面的表现非常相似在阅读次数方面。对于某些群体，如栉水母，这种条码不适合；