Hemin activation abrogates Mycoplasma hyorhinis replication in chronically infected prostate cancer cells via heme oxygenase-1 induction,FEBS Open Bio

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Hemin activation abrogates Mycoplasma hyorhinis replication in chronically infected prostate cancer cells via heme oxygenase-1 induction
FEBS Open Bio ( IF 2.8 ) Pub Date : 2021-08-10 , DOI: 10.1002/2211-5463.13271
Hanxia Huang ₁ , Alena Dabrazhynetskaya ₁ , Jacob Pluznik ₁ , Jiwen Zheng ₂ , Yong Wu ₂ , Vladimir Chizhikov ₁ , Paul W Buehler ₁ , Kenneth M Yamada ₃ , Subhash Dhawan ₁

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Mycoplasma hyorhinis (M. hyorhinis) lacks a cell wall and resists multiple antibiotics. We describe here the striking > 90% inhibitory effect of hemin, a natural inducer of the cytoprotective enzyme heme oxygenase-1 (HO-1), on M. hyorhinis replication in chronically infected LNCaP prostate cancer cells. The role of HO-1 in interrupting M. hyorhinis replication was confirmed by HO-1-specific siRNA suppression of hemin-induced HO-1 protein expression, which increased intracellular M. hyorhinis DNA levels in LNCaP cells. Proteomic analysis and transmission electron microscopy of hemin-treated cells confirmed the complete absence of M. hyorhinis proteins and intact microorganisms, respectively, strongly supporting these findings. Our study is the first to our knowledge suggesting therapeutic potential for activated HO-1 in cellular innate responses against mycoplasma infection.

中文翻译：

血红素活化通过血红素加氧酶1诱导消除慢性感染前列腺癌细胞中的猪鼻支原体复制

猪鼻支原体( M. hyorhinis ) 缺乏细胞壁并能抵抗多种抗生素。我们在此描述了血红素（一种细胞保护酶血红素加氧酶-1 (HO-1) 的天然诱导剂）对慢性感染的 LNCaP 前列腺癌细胞中的M. hyorhinis复制具有显着的 > 90% 抑制作用。HO-1 在中断M. hyorhinis复制中的作用通过 HO-1 特异性 siRNA 抑制血红素诱导的 HO-1 蛋白表达得到证实，这增加了 LNCaP 细胞中的细胞内M. hyorhinis DNA 水平。用血红素处理的细胞的蛋白质组学分析和透射电子显微镜证实完全没有M. hyorhinis蛋白质和完整的微生物分别有力地支持了这些发现。我们的研究是我们所知道的第一个表明活化的 HO-1 在针对支原体感染的细胞先天反应中具有治疗潜力的研究。

更新日期：2021-10-02

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