We realized the user-friendly and field-based point-of-care detection on a self-made portable device with the aid of personal glucose meter (PGM) for single nucleotide polymorphisms (SNPs) of melanoma and colon cancer marker BRAF V600E with good sensitivity and selectivity by integrating the loop-mediated isothermal amplification (LAMP) reaction and the strand exchange signal transduction. The device is equipped with a rotating magnetic field as well as a heating element, under which the pre-added magnetic nanochains (MNCs) help to homogenize the reaction mixture and overcome the low diffusion efficacy of the reactant and the required temperature for different reactions can be met.

中文翻译：

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基于血糖仪的超灵敏 BRAF V600E 突变检测由磁性纳米链和自制的护理点 (POC) 设备促进

我们借助个人血糖仪 (PGM) 在自制便携式设备上实现了用户友好和基于现场的即时检测，用于黑色素瘤和结肠癌标记物 BRAF V600E 的单核苷酸多态性 (SNP)，具有良好的通过整合环介导的等温扩增 (LAMP) 反应和链交换信号转导来提高灵敏度和选择性。该装置配备旋转磁场和加热元件，在加热元件下，预先添加的磁性纳米链 (MNC) 有助于使反应混合物均匀化并克服反应物的低扩散效率和不同反应所需的温度可以得到满足。