ABSTRACT

Background: Hydrogen sulfide(H₂S) is a bacterial metabolite produced as a result of bacterial growth in subgingival pockets, suggested to partake in the pathogenesis of periodontitis. H₂S has previously been shown to induce the secretion of the pro-inflammatory cytokines IL-1β and IL-18 via the NLRP3 inflammasome in monocytes.

Objective: To investigate the non-NLRP3 inflammasome-dependent immunological response of human peripheral blood mononuclear cells (PBMCs) of periodontitis patients and healthy controls exposed to H₂S in vitro.

Methods: PBMCs of periodontitis patients(N = 31) and healthy controls(N = 32) were exposed to 1 mM sodium hydrosulfide (NaHS) at 37°C for 24 h and the secretion of cytokines was compared to resting cells. TNF-α, IFN-γ, IL-6, IL-8, IL-12p40, IL-12p70, IL-17, MCP-1, and IL-1Ra secretions were measured with Bio-Plex Pro™ Human Cytokine Assay.

Results: H₂S triggered the secretion of the pro-inflammatory IFN-γ, IL-6, IL-17, TNF-α, IL-12p40, and IL-12p70, while the reverse was seen for IL-1Ra. In addition, a higher basal secretion of IFN-γ, IL-6, IL-12p70, IL-17 and MCP-1 was seen from PBMCs of periodontitis patients compared to healthy controls.

Conclusion: The bacterial metabolite H₂S triggers the secretion of pro-inflammatory cytokines from PBMCs and may thus have a prominent role in the host-bacteria interplay in periodontitis.

 抽象的

背景：硫化氢（H ₂ S）是龈袋内细菌生长产生的一种细菌代谢产物，可能参与牙周炎的发病机制。先前已证明 H ₂ S 通过单核细胞中的 NLRP3 炎症小体诱导促炎细胞因子 IL-1β 和 IL-18 的分泌。

目的：探讨体外H ₂ S暴露下牙周炎患者和健康对照人外周血单个核细胞（PBMC）的非NLRP3炎症小体依赖性免疫反应。

方法：将牙周炎患者（N = 31）和健康对照（N = 32）的PBMC在37℃下暴露于1 mM硫氢化钠（NaHS）24 h，并与静息细胞比较细胞因子的分泌。使用 Bio-Plex Pro™ 人类细胞因子测定法测量 TNF-α、IFN-γ、IL-6、IL-8、IL-12p40、IL-12p70、IL-17、MCP-1 和 IL-1Ra 分泌量。

结果： H ₂ S 触发促炎性 IFN-γ、IL-6、IL-17、TNF-α、IL-12p40 和 IL-12p70 的分泌，而 IL-1Ra 则相反。此外，与健康对照相比，牙周炎患者的 PBMC 中 IFN-γ、IL-6、IL-12p70、IL-17 和 MCP-1 的基础分泌量较高。

结论：细菌代谢产物H ₂ S触发PBMC分泌促炎细胞因子，因此可能在牙周炎中宿主-细菌相互作用中发挥重要作用。