Single-Molecule Enzymology for Diagnostics: Profiling Alkaline Phosphatase Activity in Clinical Samples,ChemBioChem

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Single-Molecule Enzymology for Diagnostics: Profiling Alkaline Phosphatase Activity in Clinical Samples
ChemBioChem ( IF 2.6 ) Pub Date : 2021-08-10 , DOI: 10.1002/cbic.202100358
Tal Gilboa _{1,

2,

3} , Alana F Ogata _{1,

2,

3} , David R Walt _{1,

2,

3}

Affiliation

Single-molecule enzymology can be used to profile heterogeneous enzyme activity in clinical samples. A diluted sample is mixed with a substrate, and the mixture is loaded to a microwell array. The change in fluorescence over time in wells containing an enzyme is used to quantify the enzyme activity, resulting in a distinct fingerprint for the target enzyme. This fingerprint is used for the quantification of multiple enzyme isotypes in the sample.