Antibiotic-resistant bacteria result in high mortality in the world. Therefore, it is necessary to find new methods as alternative antibacterial agents that decline bacterial resistance and limit the spread of serious infectious bacterial diseases. Antimicrobial photodynamic therapy (aPDT) is a non-invasive strategy against antibiotic-resistant bacteria. aPDT contains the combination of non-toxic dyes with harmless visible light to create reactive oxygen species (ROS) that selectively lead to microbial cell death. Curcumin and silver nanoparticles (AgNPs) have antibacterial properties. In this study, the aPDT with curcumin plus AgNPs as photosensitizers on planktonic and biofilm forms of Pseudomonas aeruginosa was investigated. Also, the phototoxicity effect of curcumin and AgNPs on human fibroblast cells was studied. Finally, the ROS formation and the glutathione peroxidase (GPx) activity were evaluated. The results showed that the use of curcumin in combination with AgNPs then aPDT reduced the number of bacteria in planktonic and biofilm forms. Curcumin and AgNPs did not show any significant photocytotoxic effect against human normal fibroblast. Finally, the GPx activity was decreased in presence of curcumin in combination with AgNPs then aPDT compared to control. The ROS production in curcumin plus AgNPs then aPDT was higher than the control group. Therefore, curcumin-aPDT plus AgNPs could be suggested as novel strategies in treating multi-drug-resistant bacteria such as P. aeruginosa.

抗生素抗性细菌导致世界上的高死亡率。因此，有必要寻找新的方法作为替代抗菌剂，以降低细菌耐药性并限制严重传染性细菌疾病的传播。抗菌光动力疗法 (aPDT) 是一种针对抗生素耐药细菌的非侵入性策略。aPDT 包含无毒染料与无害可见光的组合，以产生活性氧 (ROS)，选择性地导致微生物细胞死亡。姜黄素和银纳米粒子 (AgNPs) 具有抗菌特性。在这项研究中，aPDT 与姜黄素和 AgNPs 作为光敏剂对铜绿假单胞菌的浮游和生物膜形式被调查。此外，还研究了姜黄素和 AgNPs 对人成纤维细胞的光毒性作用。最后，评估了 ROS 的形成和谷胱甘肽过氧化物酶 (GPx) 的活性。结果表明，将姜黄素与 AgNPs 结合使用，然后 aPDT 减少了浮游生物和生物膜形式的细菌数量。姜黄素和 AgNPs 对人正常成纤维细胞没有显示任何显着的光细胞毒性作用。最后，与对照相比，在姜黄素与 AgNPs 结合的情况下，GPx 活性降低，然后是 aPDT。姜黄素加 AgNPs 和 aPDT 中的 ROS 产生高于对照组。因此，姜黄素-aPDT 加 AgNPs 可作为治疗铜绿假单胞菌等多重耐药菌的新策略。