A glycosaminoglycan microarray identifies the binding of SARS-CoV-2 spike protein to chondroitin sulfate E,FEBS Letters

当前位置： X-MOL 学术 › FEBS Lett. › 论文详情

Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)

A glycosaminoglycan microarray identifies the binding of SARS-CoV-2 spike protein to chondroitin sulfate E
FEBS Letters ( IF 3.0 ) Pub Date : 2021-08-10 , DOI: 10.1002/1873-3468.14173
Tomoko Watanabe ₁ , Ko Takeda ₂ , Keiko Hiemori ₁ , Toshikazu Minamisawa ₂ , Hiroaki Tateno ₁

Affiliation

Heparan sulfate (HS), a sulfated glycosaminoglycan (GAG), was reported to be a necessary host attachment factor that promotes SARS-CoV-2 infection. In this study, we developed GAG microarrays based on fluorescence detection for high-sensitivity screening of the GAG-binding specificity of proteins and applied it for the analysis of SARS-CoV-2 spike (S) protein. Among the 20 distinct GAGs, the S protein bound not only to heparin (HEP)/HS but also to chondroitin sulfate E (CSE) in a concentration-dependent manner. We then analyzed the specificity of each subunit of the S protein. While the S1 subunit showed exclusive binding to HEP, the S2 subunit also bound to CSE and HEP/HS. CSE might act as an alternative attachment factor for HS in SARS-CoV-2 infection.

中文翻译：

糖胺聚糖微阵列识别 SARS-CoV-2 刺突蛋白与硫酸软骨素 E 的结合

据报道，硫酸乙酰肝素 (HS) 是一种硫酸化糖胺聚糖 (GAG)，它是促进 SARS-CoV-2 感染的必要宿主附着因子。在本研究中，我们开发了基于荧光检测的 GAG 微阵列，用于高灵敏度筛选蛋白质的 GAG 结合特异性，并将其应用于 SARS-CoV-2 刺突 (S) 蛋白的分析。在 20 种不同的 GAG 中，S 蛋白不仅与肝素 (HEP)/HS 结合，而且以浓度依赖性方式与硫酸软骨素 E (CSE) 结合。然后我们分析了 S 蛋白每个亚基的特异性。虽然 S1 亚基显示出与 HEP 的排他性结合，但 S2 亚基也与 CSE 和 HEP/HS 结合。CSE 可能作为 SARS-CoV-2 感染中 HS 的替代附着因子。

更新日期：2021-09-27

点击分享查看原文

点击收藏

阅读更多本刊最新论文本刊介绍/投稿指南11