Bacterial infection and poor osseointegration of titanium implants are two of the main issues hindering their use in clinical applications. In order to solve these problems simultaneously, TiO₂ nanotubes were fabricated, loaded with naringin (NA) and covered by gentamicin sulfate (GS)-inserted chitosan/gelatin bilayers through layer by layer technique. The successful system of the bifunctional titanium substrate was confirmed via scanning electron microscopy, atomic force microscope, water contact angle measurement, X-ray photoelectron spectroscopy and in vitro release of NA and GS. The multilayer-coated NA-loaded TiO₂ nanotubes exhibited good biocompatibility as shown by cell morphology, cell viability assay, alkaline phosphatase activity, mineralization and qRT-PCR analysis of the osteogenesis related genes. Antibacterial experiments examining S. aureus and E. coli in vitro demonstrated that the bifunctional TiO₂ nanotubes had antibacterial capabilities. Ihis approach provides a new and feasible strategy for improving the properties of titanium implants to promote osteogenic and antibacterial activity.

钛植入物的细菌感染和骨整合不良是阻碍其在临床应用中使用的两个主要问题。为了同时解决这些问题，制备了TiO ₂纳米管，负载柚皮苷（NA）并通过逐层技术覆盖硫酸庆大霉素（GS）插入的壳聚糖/明胶双层。通过扫描电子显微镜、原子力显微镜、水接触角测量、X 射线光电子能谱和 NA 和 GS 的体外释放证实了双功能钛基底的成功体系。多层涂覆的载有 NA 的 TiO ₂纳米管表现出良好的生物相容性，如细胞形态、细胞活力测定、碱性磷酸酶活性、矿化和成骨相关基因的 qRT-PCR 分析所示。体外检查金黄色葡萄球菌和大肠杆菌的抗菌实验表明，双功能 TiO ₂纳米管具有抗菌能力。该方法为改善钛植入物的性能以促进成骨和抗菌活性提供了一种新的可行策略。