Elicitation is a biotechnological approach to improve phenolic compounds content and antioxidant properties of ready-to-eat functional foods. This study aimed to evaluate the chemical elicitation effects using salicylic acid (SA) and hydrogen peroxide (H₂O₂) in optimized-germination conditions on seedling vigor, phenolic content, and their antioxidant capacities in vitro and serum and urine of Wistar obese rats. Optimized-germination conditions of 26.5 °C and 178 h produced a 64% of germination and a sprout length of 56 mm. Only, the elicitation with H₂O₂ (20 mM) enhanced the germination (75%) and H₂O₂ (10 and 20 mM) the sprout length (69 and 59 mm, respectively). In contrast, both elicitors enhanced phenolic contents, being more significant total phenolic compounds content for SA (1 and 2 mM), up to 65.5–73.5%. SA and H₂O₂ improved total flavonoids content (36.5–64.1%), ABTS (19.3–61.1%), and DPPH capacities (51–86%), depending on SA and H₂O₂ concentration, compared with non-elicited chia sprouts. The QUENCHER antioxidant capacities of elicited chia sprouts increased up to three times more than extracts capacities, principally Q-ABTS, which could be attributed to phenolic bounds to dietary fiber. Rats fed with a high-fat and fructose diet (HFFD) and supplemented with chia sprouts, especially 1-mM SA, improve the obesity-related oxidative stress through an increase of antioxidant capacities, using DPPH and ABTS test, on serum (70–118%) and urine samples (80–116%). These results suggest that chia sprouts elicited with 1-mM SA are a source of antioxidant compounds that can be used to decrease obesity related oxidative stress.

诱导是一种生物技术方法，可提高即食功能性食品的酚类化合物含量和抗氧化特性。本研究旨在评估在优化萌发条件下使用水杨酸 (SA) 和过氧化氢 (H ₂ O ₂ )对 Wistar 肥胖大鼠幼苗活力、酚类含量及其体外抗氧化能力和血清和尿液的化学诱导作用. 26.5 °C 和 178 小时的优化发芽条件产生了 64% 的发芽率和 56 毫米的芽长。只是，H ₂ O ₂ (20 mM)的诱导增强了发芽 (75%) 和 H ₂ O ₂（10 和 20 毫米）芽长（分别为 69 和 59 毫米）。相比之下，两种诱导剂都增加了酚类物质的含量，SA（1 和 2 mM）的总酚类化合物含量更显着，高达 65.5-73.5%。SA 和 H ₂ O ₂提高了总黄酮含量 (36.5–64.1%)、ABTS (19.3–61.1%) 和 DPPH 容量 (51–86%)，具体取决于 SA 和 H ₂ O ₂浓度，与非诱导奇亚芽。引发的奇亚籽的 QUENCHER 抗氧化能力比提取物的能力增加了三倍，主要是 Q-ABTS，这可能归因于酚类与膳食纤维的结合。用高脂肪和果糖饮食 (HFFD) 喂养并补充奇亚芽，尤其是 1 mM SA 的大鼠，使用 DPPH 和 ABTS 测试，通过增加抗氧化能力来改善肥胖相关的氧化应激（70- 118%) 和尿样 (80–116%)。这些结果表明，用 1-mM SA 诱导的奇亚籽是抗氧化化合物的来源，可用于减少肥胖相关的氧化应激。