Hypericum patulum has been used as a folk medicine for its varied therapeutic effects including antifungal, wound-healing, spasmolytic, stimulant, hypotensive activities. The water decoction is drank as tea could treat cold, infantile malnutrition. The present study aims to isolate the constituents of the plant and investigate their effects on the glucose consumption in insulin-resistant HepG2 cells, furthermore, lipid metabolism in oleic acid (OA)-treated HepG2 cells was also studied. The phytochemical investigation of the plant led to the isolation of eleven compounds, and their structures were identified by spectroscopic analysis as n-dotriacontanol (1), shikimic acid (2), 1-O-caffeoylquinic acid methyl ester (3), 5-O-caffeoylquinic acid methyl ester (4), 5-O-coumaroylquinic acid methyl ester (5), 5-O-caffeoylquinic acid butyl ester (6), quercetin-3-O-α-L-rhamnoside (7), quercetin (8), quercetin-3-O-(4״-methoxy)-α-L-rahmnopyranosyl (9), hyperoside (10), and rutin (11). The results revealed that compounds 7, 9, and 10 could enhance glucose consumption significantly in hyperglycemia induced HepG2 cells and insulin-resistant HepG2 cells. In addition, the western blotting analysis result exhibited that compounds 7, 9, and 10 in high concentration (5 μM, H) group could dramatically upregulate the expression of PPARγ protein, and even the effect of them had no significant difference compared with that of rosiglitazone. Furthermore, compounds 9 and 10 in middle concentration (2.5 μM, M) group and H group could dramatically promote triglyceride metabolism and decrease TG content in OA-treated HepG2 cells, and even in H group, reactive oxygen species (ROS) level were significantly decreased compared with model group.

中文翻译：

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来自金丝桃属植物的黄酮类化合物增强葡萄糖消耗并减弱 HepG2 细胞中的脂质积累

金丝桃因其多种治疗作用而被用作民间药物，包括抗真菌、伤口愈合、解痉、兴奋剂、降压活性。喝水煎剂是因为茶可以治疗寒冷的婴儿营养不良。本研究旨在分离植物成分并研究它们对胰岛素抗性 HepG2 细胞葡萄糖消耗的影响，此外，还研究了油酸 (OA) 处理的 HepG2 细胞中的脂质代谢。该植物的植物化学研究导致分离出 11 种化合物，其结构经光谱分析鉴定为正三十烷醇 ( 1 )、莽草酸 ( 2 )、1- O-咖啡酰奎尼酸甲酯 (3 )、5- O-咖啡酰奎宁酸甲酯( 4 )、5- O-香豆酰奎尼酸甲酯( 5 )、5- O-咖啡酰奎尼酸丁酯( 6 )、槲皮素-3 - O - α - L-鼠李糖苷( 7 )、槲皮素 ( 8 )、槲皮素-3 - O- (4״-甲氧基) -α-L-吡喃吡喃糖基( 9 )、金丝桃苷 ( 10 ) 和芦丁 ( 11 )。结果表明，化合物7、9和10可以显着增加高血糖诱导的 HepG2 细胞和胰岛素抵抗的 HepG2 细胞的葡萄糖消耗。此外，蛋白质印迹分析结果显示出该化合物7,9，和10中的高浓度（5μM，H）基团可显着上调PPARγ蛋白的表达，并与比较它们的连效果没有显著差异罗格列酮。此外，化合物9和10在中等浓度（2.5 μM，M）组和H组能显着促进OA处理的HepG2细胞中甘油三酯代谢并降低TG含量，甚至在H组中，活性氧（ROS）水平也显着升高。较模型组下降。