Ralstonia solanacearum, as a causative agent of potato wilt disease, is one of the deadliest diseases worldwide, and also in the Kurdistan province, Iran. Given the importance of the disease, the aim of the present study was to isolate endophytic bacteria from potato plant tissues such as the tuber, root, stem, and leaf, to investigate their antagonistic effects on R. solanacearum. In this regard, 236 endophytic bacteria were isolated and screened in vitro. As a result, 31 isolates were found as potential antagonists against potato wilt pathogen. For phenotypic and genotypic characterization, biochemical and pathogenicity tests as well as 16S rRNA gene sequence analyses were performed. 11 isolates as representatives of different taxa were selected for further investigations. These were identified as: Bacillus pumilus Bp91, B. pumilus Bp1, B. pumilus Bp49, B. licheniformis Bl17, Paenibacillus peoriae Pa86, Pseudomonas brassicacearum Psb101, P. brassicacearum Ps169, P. putida Ps52, Chryseobacterium indologenes Ch54, C. lathyri Chl98, and Microbacterium phyllosphaerae Mi41. Notably, the maximum inhibitory effects were observed by Pseudomonas brassicacearum Psb101, P. brassicacearum Ps169, Paenibacillus peoriae Pa86, Pseudomonas putida Ps52, and Bacillus licheniformis Bl17, by forming 17.6, 17.4, 17.3, 15.5, 15.2 mm diameter inhibition zones against R. solanacearum on nutrient agar medium, respectively. Based on the results of the greenhouse test, all 11 selected isolates simultaneously reduced the disease by 27–55% and also significantly increased plant growth.

Among these, the five strains Pseudomonas brassicacearum Psb101, P. brassicacearum Ps169, P. putida Ps52, Paenibacillus peoriae Pa86, and Bacillus licheniformis Bl17 were introduced hereby as the most effective antagonists and growth enhancers under both laboratory and greenhouse conditions.

Ralstonia solanacearum作为马铃薯枯萎病的病原体，是世界上最致命的疾病之一，在伊朗库尔德斯坦省也是如此。鉴于该病害的重要性，本研究的目的是从马铃薯的块茎、根、茎和叶等植物组织中分离出内生细菌，研究它们对青枯菌的拮抗作用。对此，体外分离筛选出236株内生菌. 结果，发现 31 株分离株是马铃薯枯萎病病原体的潜在拮抗剂。对于表型和基因型表征，进行了生化和致病性测试以及 16S rRNA 基因序列分析。选择 11 个分离株作为不同分类群的代表进行进一步研究。这些被鉴定为：短小芽孢杆菌Bp91，短小芽孢杆菌BP1，短小芽孢杆菌Bp49，地衣芽孢杆菌BL17，类芽孢杆菌peoriae Pa86，假单胞菌brassicacearum Psb101，P. brassicacearum Ps169，恶臭假单胞菌PS52，金黄indologenes Ch54，C. lathyriChl98 和微杆菌phyllosphaerae Mi41。值得注意的是，通过观察到的最大抑制效应假单胞菌brassicacearum Psb101，P. brassicacearum Ps169，类芽孢杆菌peoriae Pa86，恶臭假单胞菌PS52，和地衣芽孢杆菌BL17，通过形成17.6，17.4，17.3，15.5，15.2毫米直径的抑制针对区域青枯分别在营养琼脂培养基上。根据温室试验的结果，所有 11 个选定的分离株同时将病害减少了 27-55%，并显着增加了植物生长。

其中，在实验室和温室条件下，作为最有效的拮抗剂和生长促进剂，引入了 5 个菌株Pseudomonas brasicacearum Psb101、P. brasicacearum Ps169、P. putida Ps52、Paenibacillus peoriae Pa86 和Bacillus licheniformis Bl17。