The incidence of hypoxic pulmonary hypertension (HPH) is increasing. Accumulating evidence suggests that long non-coding RNAs (lncRNAs) play an important role in HPH, but the functions and mechanism have yet to be fully elucidated. In this study, we established an HPH rat model with 8 h of hypoxia exposure (10% O2) per day for 21 days. High-throughput sequencing identified 60 differently expressed (DE) lncRNAs, 20 DE miRNAs and 695 DE mRNAs in rat lung tissue. qRT-PCR verified the accuracy of the results. Immune response, inflammatory response, leukocyte migration, cell cycle, cellular response to interleukin-1, IL-17 signalling pathway, cytokine-cytokine receptor interaction and Toll-like receptor signalling pathway were significantly enriched in DE mRNAs. According to the theory of competing endogenous RNA (ceRNA) networks, lncRNA-miRNA-mRNA network was constructed by Cytoscape software, including 7 lncRNAs, 16 miRNAs and 144 mRNAs. The results suggested that seven DE lncRNAs (Ly6l, AABR07038849.2, AABR07069008.2, AABR07064873.1, AABR07001382.1, AABR07068161.1 and AABR07060341.2) could serve as molecular sponges of the corresponding miRNAs and play a major role in HPH.

中文翻译：

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缺氧性肺动脉高压异常lncRNA-miRNA-mRNA网络的构建与分析


缺氧性肺动脉高压（HPH）的发病率正在增加。越来越多的证据表明长链非编码RNA（lncRNA）在HPH中发挥着重要作用，但其功能和机制尚未完全阐明。在本研究中，我们建立了 HPH 大鼠模型，每天缺氧暴露（10% O2）8 小时，持续 21 天。高通量测序鉴定出大鼠肺组织中 60 种不同表达 (DE) lncRNA、20 个 DE miRNA 和 695 个 DE mRNA。 qRT-PCR验证了结果的准确性。 DE mRNA 显着富集免疫反应、炎症反应、白细胞迁移、细胞周期、对白细胞介素 1 的细胞反应、IL-17 信号通路、细胞因子-细胞因子受体相互作用和 Toll 样受体信号通路。根据竞争内源RNA（ceRNA）网络理论，利用Cytoscape软件构建了lncRNA-miRNA-mRNA网络，包括7个lncRNA、16个miRNA和144个mRNA。结果表明，7个DE lncRNA（Ly6l、AABR07038849.2、AABR07069008.2、AABR07064873.1、AABR07001382.1、AABR07068161.1和AABR07060341.2）可以作为相应miRNA的分子海绵，在HPH中发挥重要作用。 。