AHNAK nucleoprotein 2 (AHNAK2) has been proposed to have an oncogenic role in various human cancers. However, the functional role of AHNAK2 in thyroid carcinoma (TC) progression has never been explored. In this study, quantitative real-time polymerase chain reaction and western blot were conducted to evaluate the expression of genes. The functional role of AHNAK2 was elucidated by cell count kit-8, colony-forming assay, wound healing assay, and Transwell invasion assay. We found that AHNAK2 was highly expressed in thyroid carcinoma, and it was tightly correlated with the pathological stage in TC. The mRNA and protein levels of AHNAK2 were increased in TC cells. Silencing of AHNAK2 restricted the proliferation, metastasis, and epithelial-mesenchymal transition (EMT) of TC cells. AHNAK2 silencing inhibited the protein expression of β-catenin and cyclin D1, and AHNAK2 overexpression had the opposite effects. Moreover, LiCl or ICG-001 exposure counteracted the effects of AHNAK2 silencing or upregulation on malignant phenotypes of TC cells. In conclusion, the knockdown of AHNAK2 restrained the proliferation, metastasis, and EMT of TC cells by inhibiting the Wnt/β-catenin pathway, providing a new potential mechanism of AHNAK2 in understanding the oncogenesis and progression of TC.

中文翻译：

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ANHAK2 的沉默通过抑制 Wnt/β-catenin 通路来限制甲状腺癌的进展。

ANHAK 核蛋白 2 (AHNAK2) 已被提议在各种人类癌症中具有致癌作用。然而，从未探索过 ANHAK2 在甲状腺癌 (TC) 进展中的功能作用。在本研究中，进行了定量实时聚合酶链反应和蛋白质印迹以评估基因的表达。通过细胞计数 kit-8、集落形成试验、伤口愈合试验和 Transwell 侵袭试验阐明了 ANHAK2 的功能作用。我们发现AHNAK2在甲状腺癌中高表达，与TC的病理分期密切相关。TC 细胞中 ANHAK2 的 mRNA 和蛋白质水平增加。ANHAK2 的沉默限制了 TC 细胞的增殖、转移和上皮间质转化 (EMT)。ANHAK2 沉默抑制了 β-catenin 和 cyclin D1 的蛋白表达，而 ANHAK2 过表达具有相反的作用。此外，LiCl 或 ICG-001 暴露抵消了 ANHAK2 沉默或上调对 TC 细胞恶性表型的影响。总之，ANHAK2的敲低通过抑制Wnt/β-catenin通路抑制了TC细胞的增殖、转移和EMT，为ANHAK2了解TC的发生和进展提供了新的潜在机制。