The safety of the probiotic strain Q180, which exerts postprandial lipid-lowering effects, was bioinformatically and phenotypically evaluated. The genome of strain Q180 was completely sequenced, and single circular chromosome of 3,197,263 bp without any plasmid was generated. Phylogenetic and related analyses using16S rRNA gene and whole-genome sequences revealed that strain Q180 is a member of Lactiplantibacillus (Lp., formerly Lactobacillus) plantarum. Antimicrobial resistance (AMR) genes were bioinformatically analyzed using all Lp. plantarum genomes available in GenBank, which showed that AMR genes are present differently depending on Lp. plantarum strains. Bioinformatic analysis demonstrated that some mobile genetic elements such as prophages and insertion sequences were identified in the genome of strain Q180, but because they did not contain harmful genes such as AMR genes and virulence factor (VF)- and toxin-related genes, it was suggested that there is no transferability of harmful genes. The minimum inhibition concentrations of seven tested antibiotics suggested by the European Food Safety Authority guidelines were slightly lower than or equal to the microbiological cut-off values for Lp. plantarum. Strain Q180 did not show hemolytic and gelatinase activities and biogenic amine-producing ability. Taken together, this study demonstrated the safety of strain Q180 in terms of absence of AMR genes and VF- and toxin-related genes as a probiotic strain.

中文翻译：

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植物乳杆菌（以前称为植物乳杆菌）Q180 的安全性评估。

对具有餐后降脂作用的益生菌菌株 Q180 的安全性进行了生物信息学和表型评估。对菌株Q180的基因组进行了完整测序，获得了3,197,263 bp的单条环状染色体，不含任何质粒。使用 16S rRNA 基因和全基因组序列的系统发育和相关分析表明，菌株 Q180 是植物乳杆菌（Lp .，以前称为乳杆菌）的成员。使用所有Lp对抗菌素抗性 (AMR) 基因进行生物信息学分析。植物油GenBank 中可用的基因组，这表明 AMR 基因的存在方式因 Lp 而异。植物菌株。生物信息学分析表明，在菌株Q180的基因组中鉴定出一些可移动的遗传元件，例如噬菌体和插入序列，但由于它们不包含有害基因，例如AMR基因和毒力因子（VF）-和毒素相关基因，所以它是表明不存在有害基因的可转移性。欧洲食品安全局指南建议的七种测试抗生素的最低抑制浓度略低于或等于Lp 的微生物临界值。植物油. 菌株 Q180 没有表现出溶血和明胶酶活性以及生物胺产生能力。综上所述，这项研究证明了 Q180 菌株作为益生菌菌株在不存在 AMR 基因和 VF 和毒素相关基因方面的安全性。