Objective. Platinum nanograss (Ptng) has been demonstrated as an excellent coating to increase the electrode roughness and reduce the impedance of microelectrodes for neural recording. However, the optimisation of the original potentiostatic electrochemical deposition (PSED) method has been performed by the original group only and no in vitro validation of functionality was reported. Approach. This study firstly reinvestigates the use of the PSED method for Ptng coating at different charge densities which highlights non-uniformities in the edges of the microelectrodes for increasing deposition charge densities, leading to a decreased impedance which is in fact an artefact. We then introduce a novel Ptng fabrication method of galvanostatic electrochemical deposition (GSED). Main results. We demonstrate that the GSED deposition method also significantly reduces the electrode impedance, raises the charge storage capacity and provides a significantly more planar electrode surface in comparison to the PSED method with negligible edge effects. In addition, we demonstrate how high-quality neural recordings were performed, for the first time, using the Ptng GSED deposition microelectrodes from human hNT neurons and how spiking and bursting were observed. Significance. Thus, the GSED Ptng deposition method presented here provides an alternative method of microelectrode fabrication for neural applications with excellent impedance and planarity of surface.

客观的。铂纳米草 (Ptng) 已被证明是一种出色的涂层，可增加电极粗糙度并降低用于神经记录的微电极阻抗。然而，原始恒电位电化学沉积 (PSED) 方法的优化仅由原始组进行，并没有报告功能性的体外验证。方法。本研究首先重新研究了 PSED 方法在不同电荷密度下 Ptng 涂层的使用，这突出了微电极边缘的不均匀性，以增加沉积电荷密度，导致阻抗降低，这实际上是一种人工制品。然后，我们介绍了一种新型的恒电流电化学沉积 (GSED) Ptng 制造方法。主要结果。我们证明，与边缘效应可忽略不计的 PSED 方法相比，GSED 沉积方法还显着降低了电极阻抗，提高了电荷存储容量，并提供了明显更平坦的电极表面。此外，我们首次展示了如何使用来自人类 hNT 神经元的 Ptng GSED 沉积微电极进行高质量的神经记录，以及如何观察到尖峰和爆裂。意义。因此，此处介绍的 GSED Ptng 沉积方法为具有出色阻抗和表面平坦度的神经应用提供了一种替代的微电极制造方法。