Glutamate (Glu) is considered the most important excitatory amino acid neurotransmitter in the mammalian Central Nervous System. Zinc (Zn) is co-released with Glu during synaptic transmission and interacts with Glutamate receptors and transporters. We performed binding experiments using [³H]MK-801 (NMDA), and [³H]Fluorowillardine (AMPA) as ligands to study Zn-Glutamate interactions in rat cortical synaptic membranes. We also examined the effects of mercury and lead on NMDA or AMPA receptors. Zinc at 1 nM, significantly potentiates [³H]MK-801 binding. Lead inhibits [³H]MK-801 binding at micromolar concentrations. At millimolar concentrations, Hg also has a significant inhibitory effect. These effects are not reversed by Zn (1 nM). Zinc displaces the [³H]FW binding curve to the right. Lead (nM) and Hg (μM) inhibit [³H]FW binding. At certain concentrations, Zn reverses the effects of these metals on [³H]FW binding. These specific interactions serve to clarify the role of Zn, Hg, and Pb in physiological and pathological conditions.

谷氨酸（Glu）被认为是哺乳动物中枢神经系统中最重要的兴奋性氨基酸神经递质。锌 (Zn) 在突触传递过程中与谷氨酸共同释放，并与谷氨酸受体和转运蛋白相互作用。我们使用 [ ³ H]MK-801 (NMDA) 和 [ ³ H]Fluorowillardine (AMPA) 作为配体进行结合实验，以研究大鼠皮质突触膜中的 Zn-谷氨酸相互作用。我们还研究了汞和铅对 NMDA 或 AMPA 受体的影响。1 nM 的锌可显着增强 [ ³ H]MK-801 结合。铅抑制 [ ³H]MK-801 在微摩尔浓度下结合。在毫摩尔浓度下，汞也具有显着的抑制作用。这些效应不会被 Zn (1 nM) 逆转。锌将 [ ³ H]FW 结合曲线向右移动。铅 (nM) 和汞 (μM) 抑制 [ ³ H]FW 结合。^{在一定浓度下，Zn 可逆转这些金属对 [ 3} H]FW 结合的影响。这些特定的相互作用有助于阐明锌、汞和铅在生理和病理条件下的作用。