Cytosolic sulfotransferase SULT1C subfamily is one of the most flexible gene subfamilies during mammalian evolution. The physiological functions of SULT1C enzymes still remain to be fully understood. In this study, common marmoset (Callithrix jacchus), a promising primate animal model, was used to investigate the functional relevance of the SULT1C subfamily. Gene database search revealed 3 intact SULT1C genes and a pseudogene in its genome. These 4 genes were named SULT1C1, SULT1C2, SULT1C3P, and SULT1C5, according to the sequence homology and gene location. Since SULT1C5 is the orthologous gene for human SULT1C2P, we propose, here, to revisit the designation of human SULT1C2P to SULT1C5P. Purified recombinant SULT1C enzymes showed sulfating activities toward a variety of xenobiotic compounds and thyroid hormones. Kinetic analysis revealed high catalytic activities of SULT1C1 and SULT1C5 for 3,3'-T2. It appears therefore that SULT1C isoforms may play a role in the thyroid hormone metabolism in common marmoset.

中文翻译：

---

催化甲状腺激素硫酸化的常见狨猴 SULT1C 亚家族成员的分子克隆和表征。


胞质磺基转移酶 SULT1C 亚家族是哺乳动物进化过程中最灵活的基因亚家族之一。 SULT1C 酶的生理功能仍有待完全了解。在本研究中，普通狨猴（Callithrix jacchus）是一种有前途的灵长类动物模型，用于研究 SULT1C 亚家族的功能相关性。基因数据库搜索发现其基因组中有 3 个完整的 SULT1C 基因和一个假基因。根据序列同源性和基因位置，这4个基因被命名为SULT1C1、SULT1C2、SULT1C3P和SULT1C5。由于 SULT1C5 是人类 SULT1C2P 的直系同源基因，因此我们在此建议重新考虑将人类 SULT1C2P 命名为 SULT1C5P。纯化的重组 SULT1C 酶对多种外源化合物和甲状腺激素表现出硫酸化活性。动力学分析显示 SULT1C1 和 SULT1C5 对 3,3'-T2 具有高催化活性。因此，SULT1C 亚型可能在普通狨猴的甲状腺激素代谢中发挥作用。