Rheum spiciforme Royle is a high value medicinal herb restricted to NW Himalayas. The medicinal properties of Rheum include anti-oxidant, anti-microbial, antitumor, anti-inflammatory, anti-fungal, anti-atherosclerotic, anti-proliferative, hepatoprotective, and immuno-enhancing. The species is threatened and endemic which demands its conservation. In this context, we have developed a premiere efficient in vitro regeneration system for this herb. The seed germination displayed phenomenal increase when transferred from soil (13.6 ± 3.1%) to half-strength Murashige and Skoog (MS) medium (92.6 ± 1.3%) fortified with 0.005 mM gibberellic acid (GA₃) and 1 mM potassium nitrate (KNO₃) and calcium chloride (CaCl₂) each with mean germination time (MGT) of 8.5 ± 1.8 d. Among four types of explants used for callusing, leaf explants responded highest with 87.3 ± 1.4% at 2 μM 2,4-dichlorophenoxyacetic acid (2,4-D) and 6-benzylaminopurine (BA) each. Nodal-segment-derived brown calluses exhibited significantly high regeneration (96.3 ± 1.6 %) at 8.0 μM BA and kinetin (KIN) each with 4.0 μM GA₃. Leaf explants observed direct somatic embryogenesis which displayed maximum (91.0 ± 3.4%) germination at 25 μM BA, 1.0 μM NAA (naphthaleneacetic acid), 2.0 μM GA₃, 50.0 μM glutamine (GTM), and adenine sulfate (ADS) each. Multiple shoot induction with mean number of 10.1 ± 2.6 shoots and elongation 4.2 ± 0.4 cm was observed at 12.5 μM BA and 0.5 μM NAA along with 25 μM GTM and ADS each. The rooted seedlings developed in half-strength liquid MS with 2.5 μM NAA were hardened and subsequently transferred to the field. The developed protocol could be utilized for various attributes which include development of large-scale micropropagation system as a conservation measure, Agrobacterium-mediated genetic transformation studies, and industrial production of important bioactive chemical constituents.

Rheum spiciforme Royle 是一种仅限于喜马拉雅西北部的高价值药草。大黄的药用特性包括抗氧化、抗微生物、抗肿瘤、抗炎、抗真菌、抗动脉粥样硬化、抗增殖、保肝和增强免疫。该物种受到威胁和地方性，需要对其进行保护。在这种情况下，我们为这种草药开发了一种首屈一指的高效体外再生系统。当从土壤 (13.6 ± 3.1%) 转移到含有 0.005 mM 赤霉酸 (GA ₃ ) 和 1 mM 硝酸钾 (KNO ₃ ) 和氯化钙 (CaCl ₂) 平均发芽时间 (MGT) 为 8.5 ± 1.8 d。在用于愈伤组织的四种外植体中，叶外植体在 2 μM 2,4-二氯苯氧乙酸 (2,4-D) 和 6-苄氨基嘌呤 (BA) 中的反应最高，为 87.3 ± 1.4%。节段衍生的棕色愈伤组织在 8.0 μM BA 和激动素 (KIN) 各具有 4.0 μM GA _{3 时}表现出显着的高再生率 (96.3 ± 1.6 %) 。叶外植体观察到直接体细胞胚胎发生，在 25 μM BA、1.0 μM NAA（萘乙酸）、2.0 μM GA _{3 下}显示出最大 (91.0 ± 3.4%) 发芽率、50.0 μM 谷氨酰胺 (GTM) 和硫酸腺嘌呤 (ADS)。在 12.5 μM BA 和 0.5 μM NAA 以及各 25 μM GTM 和 ADS 下观察到多芽诱导，平均数为 10.1 ± 2.6 芽，伸长率为 4.2 ± 0.4 cm。在含有 2.5 μM NAA 的半强度液体 MS 中发育的生根幼苗被硬化并随后转移到田间。开发的协议可用于各种属性，包括开发大规模微繁殖系统作为保护措施、农杆菌介导的遗传转化研究和重要生物活性化学成分的工业生产。