Information regarding efficient pollen viability testing methods and the most favourable pollen storage conditions for internationally important date palm cultivars is limited. No such information has been reported on Australian male selections. The in vitro pollen germination of three elite Australian male selections, “Big pod”, “Campbell” and “Tanunda”, was estimated using a modified Brewbaker and Kwack (BK) medium with combinations of various chemicals. Germination was maximized when 75 mg/l H₃BO₄, 400 mg/l Ca (NO3)2.4H₂O, 100 mg/l KNO₃, 50 mg/l MgSO₄.7H₂O and 15% (w/v) sucrose were used in a medium at pH 5.4. The viability of pollen of these three genotypes following storage in a refrigerator at 4°C was tested every 3 mo for 12 mo. Significant pollen viability was maintained after 9 mo of storage and the highest germinability (60.3%) was observed for the genotype “Tanunda”. Six tests for determining viability, including TTC (2,3,5-triphenyltetrazolium chloride), IKI (iodine potassium iodide), lactophenol cotton blue (LPCB), acetocarmine, fluorescein diacetate (FDA) and Alexander’s differential stain were evaluated and correlations between these pollen viability tests and in vitro germination were determined. A highly positive correlation between in vitro germination and the FDA viability test was observed. The metaxenic effect induced by pollinating the cultivar “Barhee” with three sources of pollen was investigated by assessing changes in the levels of malic acid, citric acid, fructose, glucose, sucrose, l-proline and myo-inositol during fruit development. The pollen source had a significant influence on the concentrations of these chemicals (metaxenic effect). Pollen of the genotype “Tanunda” significantly increased fruit size, fresh weight and seed size in comparison with other pollen sources.

关于有效的花粉活力测试方法和国际重要枣椰树品种最有利的花粉储存条件的信息是有限的。没有关于澳大利亚男性选择的此类信息的报道。在体外三个精英澳大利亚公选择花粉萌发，“大荚”，“坎贝尔”和“塔努达”，使用具有各种化学品组合的改性Brewbaker和Kwack（BK）培养基估计。当 75 mg/l H ₃ BO ₄、400 mg/l Ca (NO3)2.4H ₂ O、100 mg/l KNO ₃、50 mg/l MgSO ₄ .7H _{2 时，}发芽最大化在 pH 5.4 的培养基中使用 O 和 15% (w/v) 蔗糖。在 4°C 冰箱中储存后，这三种基因型的花粉活力每 3 个月进行一次测试，持续 12 个月。储存 9 个月后仍保持显着的花粉活力，并且观察到基因型“Tanunda”的发芽率最高（60.3%）。对确定生存力的六项测试，包括 TTC（氯化 2,3,5-三苯基四唑）、IKI（碘化钾）、乳酚棉蓝 (LPCB)、乙酰胭脂红、二乙酸荧光素 (FDA) 和亚历山大鉴别染色进行了评估，并评估了这些之间的相关性测定了花粉活力测试和体外萌发。体外高度正相关观察发芽和 FDA 活力测试。通过评估果实发育过程中苹果酸、柠檬酸、果糖、葡萄糖、蔗糖、l-脯氨酸和肌醇水平的变化，研究了用三种花粉来源对栽培品种“Barhee”授粉所诱导的代谢效应。花粉来源对这些化学物质的浓度有显着影响（metaxenic 效应）。与其他花粉来源相比，基因型“Tanunda”的花粉显着增加了果实大小、鲜重和种子大小。