Psathyrostachys huashanica Keng f. ex P.C.Kuo (2n = 2x = 14, NsNs) harbor numerous useful genes and exhibit application prospects as a breeding resource via distant hybridization. Here, a Wheat-P. huashanica alien substitution line, derived from common wheat 7182 and P. huashanica, was characterized by Multiple experimental evidences. The cytological results showed the chromosome number of the line was 42. Genomic in situ hybridization and fluorescence in situ hybridization (GISH-FISH) analysis indicated the line had a pair of chromosomes Ns from P. huashanica, and 40 chromosomes from common wheat. Verification using expressed sequence tag–sequence tagged site (EST-STS) (BE497584, BE443796, BG313767), polymerase chain reaction (PCR)-based landmark unique gene (PLUG) (TNAC1026-HaeIII, TNAC1043-TaqI, TNAC1091-HaeIII) and high molecular weight glutenin subunit (HMW-GS) markers confirmed that a pair of wheat chromosomes 1D in the line were substituted by a pair of P. huashanica chromosomes 1Ns. Therefore, we can conclude that the alien chromosome line was a wheat- P. huashanica 1Ns(1D) disomic substitution line and named as wheat-Ph1D. In comparison to the common wheat parent 7182, agronomic trait evaluations showed that wheat-Ph1D exhibited significant improvements, including plant height, grain length, thousand-grain weight, the contents of crude protein and gluten protein, starch content, dough stability time and resistant to stripe rust at the adult stage. Consequently, wheat-Ph1D could be a potential bridge material in wheat breeding with specific agronomic traits.

华山新春藤f. ex PCKuo (2n = 2x = 14, NsNs) 包含许多有用的基因，并通过远缘杂交显示出作为育种资源的应用前景。在这里，从普通小麦 7182 和P. huashanica 中衍生出的小麦-P . huashanica外来替代品系通过多项实验证据进行表征。细胞学结果显示该品系的染色体数为42条。基因组原位杂交和荧光原位杂交(GISH-FISH)分析表明该品系有一对来自华山青霉的染色体Ns和来自普通小麦的40条染色体。使用表达序列标签-序列标记位点 (EST-STS) 进行验证（BE497584、BE443796、BG313767)、基于聚合酶链反应 (PCR) 的标志性独特基因 (PLUG)（TNAC1026-HaeIII、TNAC1043-TaqI、TNAC1091-HaeIII）和高分子量麦谷蛋白亚基 (HMW-GS) 标记证实了一对小麦染色体 1D该品系被一对华山假单胞菌染色体1Ns取代。因此，我们可以得出结论，外来染色体系是小麦-华山青霉1Ns(1D)二体置换系，命名为wheat-Ph1D。与普通小麦亲本7182相比，农艺性状评价表明，小麦-Ph1D表现出显着的改善，包括株高、粒长、千粒重、粗蛋白和面筋蛋白含量、淀粉含量、面团稳定时间和抗性。在成虫阶段去除条锈病。因此，小麦-Ph1D 可能成为具有特定农艺性状的小麦育种的潜在桥梁材料。