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A screening method for decoloration of xenobiotic dyes by fungi
Journal of Microbiological Methods ( IF 1.7 ) Pub Date : 2021-08-10 , DOI: 10.1016/j.mimet.2021.106301
Brigit van Brenk 1 , Han A B Wösten 1
Affiliation  

Wood degrading fungi are often screened for their ability to degrade xenobiotics such as dyes. Dye decoloration by these fungi on solid media could until now only be assessed qualitatively. We here describe a fast quantitative method to screen for dye decoloration on such media. Decoloration of crystal violet (CV), malachite green (MG), orange G (OG), rose bengal (RB) and remazol brilliant blue R (RBBR) by 124 isolates of the basidiomycete Schizophyllum commune was quantified with a flatbed scanner and the CIE-L*a*b* model. Colour and intensity changes were calculated with the Euclidean distance formula. More than 10 strains showed high MG decoloration. Isolates 136, 140 and 213 showed superior CV decoloration, while OG was most effectively decolorized by isolates 183, 216 and 227. Six strains showed high RB decoloration with isolate 216 being superior. The latter strain was also highly active on RBBR together with isolates 177 and 227. Together, dye decoloration was highly variable between the 124 isolates but strain 216 showed high activity on 3 out of 5 dyes. The fast screening method described in this paper enables identification of strains effectively decolorizing dyes.



中文翻译:

一种真菌对异源染料脱色的筛选方法

木材降解真菌通常会根据它们降解异生物质(如染料)的能力进行筛选。这些真菌在固体培养基上造成的染料脱色到目前为止只能进行定性评估。我们在这里描述了一种快速定量方法来筛选此类介质上的染料脱色。124 株担子菌Schizophyllum 公社对结晶紫 (CV)、孔雀石绿 (MG)、橙 G (OG)、玫瑰红 (RB) 和雷马唑亮蓝 R (RBBR) 的脱色用平板扫描仪和 CIE-L*a*b* 模型量化。使用欧几里得距离公式计算颜色和强度变化。超过10个菌株表现出高MG脱色。分离株 136、140 和 213 表现出优异的 CV 脱色,而分离株 183、216 和 227 对 OG 的脱色最有效。六株菌株表现出高 RB 脱色,其中分离株 216 表现优异。后一种菌株与分离株 177 和 227 一起对 RBBR 也具有高活性。一起,124 种分离株之间的染料脱色变化很大,但菌株 216 对 5 种染料中的 3 种表现出高活性。本文中描述的快速筛选方法能够识别有效脱色染料的菌株。

更新日期:2021-08-12
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