Abstract

Fungal endophytes have been recorded in various plant species with a richness of diversity, and their presence plays an essential role in host plant protection against biotic and abiotic stresses. This study applied the Illumina MiSeq sequencing platform based on the amplification of fungal ribosomal ITS2 region to analyze fungal endophytic communities of two oak species (Quercus mongolica and Q. serrata) with different oak wilt disease susceptibilities in Korea. The results showed a total of 230,768 sequencing reads were obtained and clustered at a 97% similarity threshold into 709 operational taxonomic units (OTUs). The OTUs of Q. serrata were higher than that of Q. mongolica with the number of 617 OTUs and 512 OTUs, respectively. Shannon index also showed that Q. serrata had a significantly higher level of fungal diversity than Q. mongolica. Total of OTUs were assigned into 5 fungal phyla, 17 classes, 60 orders, 133 families, 195 genera, and 280 species. Ascomycota was the dominant phylum with 75.11% relative abundance, followed by Basidiomycota with 5.28%. Leptosillia, Aureobasidium and Acanthostigma were the most abundant genera detected in Q. serrata with the average relative abundance of 2.85, 2.76, and 2.19%, respectively. On the other hand, Peltaster, Cladosporium and Monochaetia were the most common genera detected in Q. mongolica with the average relative abundance of 4.83, 3.03, and 2.87%, respectively. Our results indicated that fungal endophytic communities were significantly different between two oak species and these differences could influence responses of host trees to oak wilt disease caused by Raffaelea quercus-mongolicae.

摘要

真菌内生菌已被记录在具有丰富多样性的各种植物物种中，它们的存在在宿主植物抵抗生物和非生物胁迫的保护中起着至关重要的作用。本研究应用基于真菌核糖体 ITS2 区域扩增的 Illumina MiSeq 测序平台，分析了韩国具有不同橡树枯萎病易感性的两种橡树种（Quercus mongolica和Q. serrata）的真菌内生群落。结果显示总共获得了 230,768 个测序读数，并以 97% 的相似性阈值聚类为 709 个操作分类单元 (OTU)。Q. serrata的OTUs高于Q. mongolicaOTU 数量分别为 617 个和 512 个。香农指数还表明，锯缘青蟹的真菌多样性水平明显高于蒙古青蟹。OTUs共分为5个真菌门、17纲、60目、133科、195属、280种。子囊菌门是优势门，相对丰度为 75.11%，其次是担子菌门，占 5.28%。Leptosillia、Aureobasidium和Acanthostigma是Q. serrata 中检测到的最丰富的属，平均相对丰度分别为2.85、2.76和 2.19%。另一方面，Peltaster、Cladosporium和Monochaetia是在Q. mongolica 中检测到的最常见的属，平均相对丰度分别为 4.83、3.03 和 2.87%。我们的研究结果表明，两种橡树种之间的真菌内生群落存在显着差异，这些差异会影响寄主树木对Raffaelea quercus-mongolicae引起的橡树枯萎病的反应。