Bone marrow mesenchymal stem cells (BMSCs) are intraosseous stem cells, and the effects of tensile strain on BMSC differentiation mediate several bone-related treatments. To study the response of BMSCs under tension, we designed and developed a small cellular tension instrument, iStrain. When iStrain applied tension on BMSCs, these cells exhibited convergence in the alignment direction and lengthening of the cell processes and cell body. Real-time quantitative polymerase chain reaction (RT-qPCR) and western blotting demonstrated that iStrain-mediated cyclic tension promotes the differentiation of BMSCs toward osteogenesis and fibrogenesis. And the mRNA and protein expression of differentiation-related genes changes with the extension of tension time.

中文翻译：

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一种新型张力机通过循环张力促进骨髓间充质干细胞成骨和成纤维细胞分化

骨髓间充质干细胞 (BMSCs) 是骨内干细胞，拉伸应变对 BMSC 分化的影响介导了几种骨相关治疗。为了研究 BMSCs 在张力下的反应，我们设计并开发了一种小型细胞张力仪 iStrain。当 iStrain 对 BMSCs 施加张力时，这些细胞在排列方向上表现出收敛和细胞突起和细胞体的延长。实时定量聚合酶链反应 (RT-qPCR) 和蛋白质印迹证明 iStrain 介导的循环张力促进 BMSCs 向成骨和纤维化分化。并且分化相关基因的mRNA和蛋白表达随着张力时间的延长而变化。