Enzymes from phytopathogenic fungi are desirable for biotechnological applications and a highly virulent phytopathogen shows great appeal for enzymes production. To assess the biotechnological potential of Kretzschmaria zonata, a plant pathogenic fungus, we analyzed its enzymatic profile after growth on six different types of lignocellulosic biomasses. The fungus was able to produce a wide variety of enzymes with superior xylanase activity. The corn cob induced the highest specific activity of xylanase, 56.30 U/mg of protein, as well as other important enzymatic activities such as endoglucanase, 11.20 U/mg of protein; pectinase, 4.52 U/mg of protein; and β-glucosidase, 2.77 U/mg of protein. The highest release of xylose, 0.88 g/L, was observed after saccharification of 10% of alkaline pretreated sugarcane bagasse by a commercial cocktail supplemented with the crude extract from K. zonata after growth on corn cob. The fungus extract is rich in hemicellulases and accessory enzymes and the result showed synergism between the enzymes present in the commercial mixture and in the K. zonata extract. This is the first report concerning the biotechnological potential of the fungus K. zonata, especially regarding to its ability to produce plant biomass degrading enzymes related to second generation ethanol production.

来自植物病原真菌的酶对于生物技术应用来说是理想的，并且高毒力植物病原体对酶的生产显示出极大的吸引力。评估Kretzschmaria zonata的生物技术潜力，一种植物病原真菌，我们分析了其在六种不同类型的木质纤维素生物质上生长后的酶谱。该真菌能够产生多种具有优异木聚糖酶活性的酶。玉米芯诱导木聚糖酶的比活性最高，为56.30 U/mg蛋白质，以及其他重要的酶活性如内切葡聚糖酶，为11.20 U/mg蛋白质；果胶酶，4.52 U/mg 蛋白质；和 β-葡萄糖苷酶，2.77 U/mg 蛋白质。木糖的最高释放量为 0.88 g/L，在用商业鸡尾酒糖化 10% 的碱性预处理甘蔗渣后，辅以K. zonata粗提物在玉米芯上生长后。真菌提取物富含半纤维素酶和辅助酶，结果表明商业混合物中存在的酶和K. zonata提取物中存在的酶之间存在协同作用。这是关于真菌K. zonata的生物技术潜力的第一份报告，特别是关于其生产与第二代乙醇生产相关的植物生物质降解酶的能力。