Actinobacteria is a phylum composed of aerobic, Gram-positive, and filamentous bacteria with a broad spectrum of biological activity, including antioxidant, antitumor, and antibiotic. The crude extract of Streptomyces griseocarneus R132 was fractionated on a C18 silica column and the isolated compound was identified by ¹H and ¹³C nuclear magnetic resonance as 3-(phenylprop-2-enoic acid), also known as trans-cinnamic acid. Antimicrobial activity against human pathogens was assayed in vitro (disk-diffusion qualitative test) and in vivo using Galleria mellonella larvae (RT-qPCR). The methanol fractions 132-F30%, 132-F50%, 132-F70%, and 132-F100% inhibited the Escherichia coli (ATCC 25922) and Staphylococcus aureus (MRSA) growth in vitro the most effectively. Compared with the untreated control (60–80% of larvae death), the fractions and isolated trans-cinnamic acid increased the survival rate and modulated the immune system of G. mellonella larvae infected with pathogenic microorganisms. The anti-infection effect of the S. griseocarneus R132 fermentation product led us to sequence its genome, which was assembled and annotated using the Rast and antiSMASH platforms. The assembled genome consisted of 227 scaffolds represented on a linear chromosome of 8.85 Mb and 71.3% of GC. We detected conserved domains typical of enzymes that produce molecules with biological activity, such as polyketides and non-ribosomal and ribosomal peptides, indicating a great potential for obtaining new antibiotics and molecules with biotechnological application.

放线菌是由需氧菌、革兰氏阳性菌和丝状菌组成的门，具有广泛的生物活性，包括抗氧化、抗肿瘤和抗生素。Streptomyces griseocarneus R132的粗提物在 C18 硅胶柱上分离，分离的化合物通过¹ H 和¹³ C 核磁共振鉴定为 3-(苯基丙-2-烯酸)，也称为反式肉桂酸。在体外（圆盘扩散定性试验）和体内使用梅隆氏菌幼虫 (RT-qPCR) 测定了对人类病原体的抗菌活性。甲醇馏分 132-F30%、132-F50%、132-F70% 和 132-F100% 抑制大肠杆菌(ATCC 25922) 和金黄色葡萄球菌(MRSA) 的体外生长最有效。与未经处理的对照（60-80% 的幼虫死亡）相比，这些组分和分离的反式肉桂酸提高了感染病原微生物的G. mellonella幼虫的存活率并调节了免疫系统。S. griseocarneus R132 发酵产物的抗感染作用使我们对其基因组进行了测序，该基因组是使用 Rast 和 antiSMASH 平台组装和注释的。组装的基因组由 227 个支架组成，代表在 8.85 Mb 的线性染色体和 71.3% 的 GC 上。我们检测到产生具有生物活性的分子的酶的典型保守结构域，例如聚酮化合物和非核糖体和核糖体肽，表明获得具有生物技术应用的新抗生素和分子的巨大潜力。