Whether subclinical shedding of canine parvovirus (CPV) by cats might contribute to the epidemiology of canine CPV infections, particularly in facilities housing both cats and dogs, requires clarification. Conflicting results are reported to date. Using conventional PCR (cPCR) to amplify the VP2 gene, shedding of the CPV variants (CPV-2a, 2b, 2c) by healthy cats in multi-cat environments was reportedly common in Europe but rare in Australia. The aim of this study was to determine whether low-level faecal CPV shedding occurs in multi-cat environments in Australia and Italy using a TaqMan real-time PCR to detect Carnivore protoparvovirus 1 (CPV and feline parvovirus, FPV) DNA, and minor-groove binder probe real-time PCR assay to differentiate FPV and CPV types and to characterize CPV variants. In total, 741 non-diarrhoeic faecal samples from shelters in Australia (n = 263) and from shelters or cat colonies in Italy (n = 478) were tested. Overall, Carnivore protoparvovirus 1 DNA was detected in 49 of 741 (6.61 %) samples. Differentiation was possible for 31 positive samples. FPV was most common among positive samples (28/31, 90.3 %). CPV was detected in 4/31 samples (12.9 %) including CPV-2a in one sample, CPV-2b in another and co-infections of FPV/CPV-2b and CPV-2a/CPV-2b in the remaining two samples. A high rate of subclinical FPV infection was detected in one shelter during an outbreak of feline panleukopenia, during which 21 of 22 asymptomatic cats (95.5 %) sampled were shedding FPV. Faecal shedding of CPV by cats in multi-cat environments is uncommon suggesting that domestic cats are not significant reservoirs of CPV.

猫对犬细小病毒 (CPV) 的亚临床脱落是否可能导致犬 CPV 感染的流行病学，特别是在同时饲养猫和狗的设施中，需要澄清。迄今为止，报告的结果相互矛盾。使用常规 PCR (cPCR) 扩增 VP2 基因，据报道，健康猫在多猫环境中脱落 CPV 变体（CPV-2a、2b、2c）在欧洲很常见，但在澳大利亚很少见。本研究的目的是使用 TaqMan 实时 PCR 检测食肉动物原细小病毒 1 型，以确定澳大利亚和意大利的多猫环境中是否发生低水平的粪便 CPV 脱落。（CPV 和猫细小病毒，FPV）DNA 和小沟结合剂探针实时 PCR 检测，以区分 FPV 和 CPV 类型并表征 CPV 变体。总共测试了来自澳大利亚庇护所（n = 263）和意大利庇护所或猫群（n = 478）的 741 个非腹泻粪便样本。总体而言，食肉动物原细小病毒 1在 741 个样本中的 49 个（6.61%）中检测到 DNA。31 个阳性样本可以区分。FPV 在阳性样本中最为常见 (28/31, 90.3 %)。在 4/31 个样品中检测到 CPV（12.9%），包括一个样品中的 CPV-2a、另一个样品中的 CPV-2b 以及其余两个样品中 FPV/CPV-2b 和 CPV-2a/CPV-2b 的混合感染。在猫泛白细胞减少症爆发期间，在一个收容所检测到亚临床 FPV 感染率很高，在此期间采样的 22 只无症状猫中有 21 只（95.5%）正在脱落 FPV。在多猫环境中，猫通过粪便排出 CPV 并不常见，这表明家猫不是 CPV 的重要宿主。