Echinococcus multilocularis metacestodes mainly reside in liver in humans and animals, and cause serious damages. UBE2N was herein shown to be downregulated in response to the infection. UBE2N was further shown to be predominantly expressed in the hepatocytes, which was also significantly downregulated during the infection. UBE2N was a target of emu-miR-4989, which was loaded into the exosomes secreted by parasites. These emu-miR-4989-encapsulating exosomes were internalized by hepatocytes, and induced a significant decrease of relative luciferase activity in the cells transfected with the construct containing a wild type of UBE2N 3’-UTR compared to the control (p < 0.05). These results demonstrate that emu-miR-4989 is involved in the UBE2N inhibition in the hepatocytes during E. multilocularis through exosomes.

多房棘球绦虫主要存在于人和动物的肝脏中，危害严重。UBE2N 在本文中显示为响应感染而下调。UBE2N 进一步显示主要在肝细胞中表达，在感染期间也显着下调。UBE2N 是 emu-miR-4989 的靶标，它被加载到寄生虫分泌的外泌体中。这些包裹着 emu-miR-4989 的外泌体被肝细胞内化，并在用含有野生型 UBE2N 3'-UTR 的构建体转染的细胞中诱导相对荧光素酶活性显着降低（p < 0.05）。这些结果表明，emu-miR-4989 参与了肝细胞中 UBE2N 的抑制。E. multilocularis通过外泌体。