Global warming has led to severe drought conditions. The selection of plant varieties that can withstand drought and produce increased yields are of utmost importance. In the current study, secondary metabolites, seed trait and fiber characteristic of cottonseeds (Gossypium hirsutum) exposed to double and third water stress exposure was investigated. Total phenol and tannin content in W₁S₃₃ increased significantly after third water stress exposure. Accumulation of wax was enhanced in seeds of W₃S₃₃ and W₃S₃₄ that were subjected to third water stress. Fiber quality parameters decreased when cottonseeds were rainfed. High irrigation resulted in fragile and delicate fiber. Seeds grown under 66% FC irrigation saved water and produced seeds that had the potential of producing high quality fibers. In silico analysis was performed on cellulose synthase A (CesA) and xyloglucan endotransglycosylase (XET) enzymes present in Gossypium hirsutum. The intracellular locations of the CesA and XET1 enzymes are the plasma membrane and cell wall, respectively. Proline is conserved in the C-terminal of the CesA enzyme and plays an important role in enzyme functionality. This study provides a better understanding as to the mechanisms by which the plant can tolerate and combat water stress conditions as well as reduce water consumption. In order to grow cotton seeds with desirable morphometric characteristics and optimal fibers under water stress exposure and in dry areas, it is better to use seeds that are irrigated under optimal irrigation conditions, ie 66% FC.

全球变暖导致了严重的干旱状况。选择能够抵御干旱并提高产量的植物品种至关重要。在目前的研究中，研究了暴露于双重和三次水分胁迫下的棉籽（ Gossypium hirsutum ）的次生代谢物、种子性状和纤维特性。_{在第三次水分胁迫暴露后，W 1} S ₃₃中总酚和单宁含量显着增加。_{W 3} S ₃₃和 W ₃ S ₃₄种子中蜡的积累增强遭受第三次水分胁迫。棉籽雨育时纤维质量参数下降。高灌溉导致脆弱和脆弱的纤维。在 66% FC 灌溉下生长的种子节约了水，并生产出具有生产高质量纤维潜力的种子。对陆地棉中存在的纤维素合酶 A (CesA) 和木葡聚糖内转糖基化酶 (XET) 进行了计算机分析。CesA 和 XET1 酶的细胞内位置分别是质膜和细胞壁。脯氨酸在 CesA 酶的 C 末端是保守的，在酶功能中起重要作用。这项研究提供了对植物耐受和对抗水分胁迫条件以及减少用水量的机制的更好理解。为了在水分胁迫下和干旱地区种植具有理想形态特征和最佳纤维的棉花种子，最好使用在最佳灌溉条件下灌溉的种子，即 66% FC。