Antimicrobial resistance is not restricted to clinics but also spreading fast in the aquatic environment. This study focused on the prevalence and diversity of extended-spectrum β-lactamase (ESBL) genes among bacteria from lentic and effluent water in Delhi-NCR, India. Phenotypic screening of 436 morphologically distinct bacterial isolates collected from diverse sites revealed that 106 (∼24%) isolates were ESBL positive. Antibiotic profiling showed that 42, 60, 78 and 59% ESBL producing isolates collected from Ghazipur slaughterhouse, Lodhi garden pond, Hauz Khas lake and Jasola wastewater treatment plant, respectively, were multidrug-resistant (MDR). The multiple antibiotic resistance (MAR) index varied from 0.20 to 0.32 among selected locations. The prevalence of ESBL gene variants blaSHV, blaTEM and blaCTX-M were found to be 17.64, 35.29 and 64%, respectively. Furthermore, the analysis of obtained gene sequences showed three variants of blaCTX-M (15, 152 and 205) and two variants of blaTEM (TEM-1 and TEM-116) among ESBL producers. The co-existence of 2–3 gene variants was recorded among 48% ESBL positive isolates. New reports from this study include the blaCTX-M gene in Acinetobacter lwoffii, Enterobacter ludwigii, Exiguobacterium mexicanum and Aeromonas caviae. Furthermore, the identification of blaTEM and blaSHV in an environmental isolate of A. caviae is a new report from India.

抗菌素耐药性不仅限于诊所，而且在水生环境中也迅速蔓延。这项研究的重点是来自印度德里 NCR 的静水和流出水中的细菌中超广谱β-内酰胺酶 (ESBL) 基因的流行和多样性。从不同地点收集的 436 株形态不同的细菌分离株的表型筛选显示，106 株（~24%）分离株为 ESBL 阳性。抗生素分析表明，分别从 Ghazipur 屠宰场、Lodhi 花园池塘、Hauz Khas 湖和 Jasola 废水处理厂收集的产 ESBL 分离株的产率分别为 42%、60%、78% 和 59%，具有多重耐药性 (MDR)。选定地点的多重抗生素耐药性 (MAR) 指数从 0.20 到 0.32 不等。ESBL 基因变异bla的流行发现 SHV、bla TEM 和bla CTX-M 分别为 17.64、35.29 和 64%。此外，对获得的基因序列的分析表明，ESBL 生产者中存在bla CTX-M 的三种变体（15、152和 205）和两种bla TEM变体（TEM-1 和 TEM-116）。在 48% ESBL 阳性分离株中记录了 2-3 个基因变异的共存。从这项研究的新报告包括喇嘛在CTX-M基因的鲍曼不动lwoffii，肠杆菌属ludwigii，微小杆菌属mexicanum和气单胞菌的豚鼠。此外，在环境分离株中鉴定bla TEM 和bla SHVA. caviae是来自印度的新报告。