Al-Zarqa River is the second main tributary to River Jordan after the Yarmouk River. The river flow has been modified by discharge of industrial wastewater and treated domestic water. Concerns about the occurrence of waterborne pathogenic viruses in the surface waters of Al-Zarqa River prompted the analysis of the surface water quality with respect to the presence of enteric viruses. Viruses were concentrated from a total of 33 different water environmental samples including raw sewage, effluent samples and river water collected from and around the river over a period of 11 months. Calculated recovery yields for these concentration methods ranged between 2 and 8%. Polymerase chain reaction (PCR), reverse transcriptase-PCR (RT-PCR), nested RT-PCR and southern blotting hybridization analysis were used for the detection of hepatitis A virus, norovirus, astrovirus and human adenovirus 40/41, with the later one being detected in 21 (64%) of the samples that also showed previous positive presence for enteroviruses. To our knowledge, this is the first molecular biology report in Jordan describing the circulation of adenoviruses, which were detected more frequently than enteroviruses in sewage and water samples, and therefore, they can be used as an index for the presence of human pathogenic viruses in water environment.

Al-Zarqa 河是继耶尔穆克河之后约旦河的第二条主要支流。通过排放工业废水和处理过的生活用水，河流流量得到了改变。对 Al-Zarqa 河地表水中出现水源性致病病毒的担忧促使对地表水水质进行了肠道病毒存在的分析。在 11 个月的时间里，病毒从总共 33 个不同的水环境样本中浓缩，包括未处理的污水、污水样本和从河流及其周围收集的河水。这些浓缩方法的计算回收率在 2% 到 8% 之间。使用聚合酶链反应（PCR）、逆转录酶-PCR（RT-PCR）、巢式RT-PCR和Southern印迹杂交分析检测甲型肝炎病毒，诺如病毒、星状病毒和人类腺病毒 40/41，后者在 21 个（64%）的样本中检测到，这些样本之前也显示肠道病毒呈阳性。据我们所知，这是约旦第一份描述腺病毒循环的分子生物学报告，腺病毒在污水和水样中的检测频率高于肠道病毒，因此，它们可以作为人类致病病毒存在的指标。水环境。