Optical-resolution photoacoustic microscopy (OR-PAM) combines high spatial resolution and strong absorption-based contrast in tissue, which has enabled structural and spectroscopic imaging of endogenous chromophores, primarily hemoglobin. Conventional piezoelectric ultrasound transducers are typically placed far away from the photoacoustic source due to their opacity, which reduces acoustic sensitivity. Optical ultrasound sensors are an alternative as their transparency allows them to be positioned close to the sample with minimal source-detector distances. In this work, a backward-mode OR-PAM system based on a planar Fabry-Pérot ultrasound sensor and coaxially aligned excitation and interrogation beams was developed. Two 3D imaging modes, using raster-scanning for enhanced image quality and continuous-scanning for fast imaging, were implemented and tested on a leaf skeleton phantom. In fast imaging mode, a scan-rate of 100,000 A-lines/s was achieved. 3D images of a zebrafish embryo were acquired The transparency of the FP sensor in the visible and near-infrared wavelength region makes it suitable for combined functional and molecular imaging applications using OR-PAM and multi-photon fluorescence microscopy.

中文翻译：

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使用平面法布里-珀罗传感器进行 3D 成像的后向模式光学分辨率光声显微镜

光学分辨率光声显微镜 (OR-PAM) 结合了组织中的高空间分辨率和基于吸收的强对比度，从而能够对内源生色团（主要是血红蛋白）进行结构和光谱成像。传统的压电超声换能器由于其不透明性通常放置在远离光声源的位置，这会降低声学灵敏度。光学超声传感器是一种替代方案，因为其透明度使它们能够以最小的源检测器距离放置在靠近样品的位置。在这项工作中，开发了一种基于平面法布里-珀罗超声传感器和同轴对准的激励和询问光束的后向模式 OR-PAM 系统。在叶子骨架模型上实施并测试了两种 3D 成像模式，即使用光栅扫描来增强图像质量和使用连续扫描来快速成像。在快速成像模式下，扫描速率达到 100,000 A 线/秒。获取斑马鱼胚胎的 3D 图像 FP 传感器在可见光和近红外波长区域的透明度使其适合使用 OR-PAM 和多光子荧光显微镜进行功能和分子成像组合应用。