It suggests that the poly (3-hydroxybutyric acid-co-3-hydroxyvaleric acid) (PHBV) scaffold can be used for cartilage tissue engineering, but PHBV is short of bioactivity that is required for cartilage regeneration. To fabricate a bioactive cartilage tissue engineering scaffold that promotes cartilage regeneration, quercetin (QUE) modified PHBV (PHBV-g-QUE) fibrous scaffolds were prepared by a two-step surface modification method. The PHBV-g-QUE fibrous scaffold facilitates the growth of chondrocytes and maintains chondrocytic phenotype resulting from the upregulation of SOX9, COL II, and ACAN. The PHBV-g-QUE fibrous scaffold inhibited apoptosis of chondrocyte and reduced oxidative stress of chondrocytes by regulating the transcription of related genes. Following PHBV-g-QUE fibrous scaffolds and PHBV fibrous scaffolds with adhered chondrocytes were implanted into nude mice for 4 weeks, it demonstrated that PHBV-g-QUE fibrous scaffolds significantly promoted cartilage regeneration compared with the PHBV fibrous scaffolds. Hence, it suggests that the PHBV-g-QUE fibrous scaffold can be potentially applied in the clinical treatment of cartilage defects in the future.

这表明聚（3-羟基丁酸-co-3-羟基戊酸）（PHBV）支架可用于软骨组织工程，但PHBV缺乏软骨再生所需的生物活性。为了制备促进软骨再生的生物活性软骨组织工程支架，采用两步表面修饰法制备了槲皮素（QUE）修饰的 PHBV（PHBV-g-QUE）纤维支架。PHBV-g-QUE 纤维支架促进软骨细胞的生长并维持由 SOX9、COL II 和 ACAN 上调引起的软骨细胞表型。PHBV-g-QUE纤维支架通过调节相关基因的转录抑制软骨细胞凋亡，降低软骨细胞氧化应激。将PHBV-g-QUE纤维支架和附着软骨细胞的PHBV纤维支架植入裸鼠4周后，表明与PHBV纤维支架相比，PHBV-g-QUE纤维支架显着促进软骨再生。因此，这表明 PHBV-g-QUE 纤维支架在未来可潜在地应用于软骨缺损的临床治疗。