Abstract

Probiotics are potential health-promoting microorganisms representing a significant health aid with diverse pharmaceutical applications. But its role in modulating the human gut microbiome necessities a profound analysis. Undoubtedly, the human gut is a reservoir of infinite species such as bacteria, viruses, fungi, etc. But host with chronic illness bids enrichment of the pathogenic strain which may alter the composition gut microbiome. Culture-based techniques are providing unclear taxonomic data where the possibility of the discrepancy is very high. Therefore the use of next-generation sequencing (NGS) analysis helps to explore depth understanding of the curd microbiome and can generate big genomic data. The present investigation aims to explore the microbial diversity of curd samples from different local dairy farms of Odisha, India using a metagenomic approach. The data read comprising 304,725 sequences totaling 148,546,295 base pairs were interpreted. Taxonomical distribution at different levels unfolded the absolute abundance of phylum, class, order, family, genus, and species within each microbial community. The most dominant species found to be Lactobacillus at order level is 49%, whereas phyla proteobacteria and firmicutes have shown taxonomic percentage abundance of 50.01% and 48.77%, respectively. However, nearly 26.41% of unclassified species from the Lactococcus genus were identified in the sample at different taxonomic levels. The metagenomics sequence data has been deposited to sequence read archive (SRA) of NCBI database under Bioproject PRJNA383620. The present study has vital implications in understanding the microbial diversity in curd samples with their role in human health and application in future pharmaceutical probiotic research.

摘要

益生菌是潜在的促进健康的微生物，代表着具有多种药物应用的重要健康援助。但它在调节人类肠道微生物组中的作用需要深入分析。毫无疑问，人类肠道是细菌、病毒、真菌等无限物种的储存库。但患有慢性疾病的宿主会增加致病菌株，这可能会改变肠道微生物组的组成。基于文化的技术正在提供不明确的分类数据，其中差异的可能性非常高。因此，使用下一代测序 (NGS) 分析有助于探索对凝乳微生物组的深入了解，并可以生成大的基因组数据。本调查旨在探索来自奥里萨邦不同当地奶牛场的凝乳样品的微生物多样性，印度使用宏基因组方法。解释了包含 304,725 个序列、总计 148,546,295 个碱基对的数据读取。不同层次的分类学分布展现了每个微生物群落内门、纲、目、科、属和种的绝对丰度。发现的最主要的物种是目水平的乳酸杆菌为 49%，而变形菌门和厚壁菌门的分类学百分比丰度分别为 50.01% 和 48.77%。然而，在不同分类水平的样本中发现了近 26.41% 的乳球菌属未分类物种。宏基因组序列数据已保存在 Bioproject PRJNA383620 下的 NCBI 数据库的序列读取档案 (SRA) 中。本研究对于了解凝乳样品中的微生物多样性及其在人类健康中的作用以及在未来药物益生菌研究中的应用具有重要意义。