Abstract

In the present study, candidate single nucleotide polymorphism (SNP) g.92450765 G > A of leptin gene was explored for Bos indicus cattle with an aim to explore its possible effect on production and reproduction traits. The genotypic and allelic frequencies of BsaA 1 genotyped SNP g.92450765 G > A indicated the predominance of AG (0.65) genotyped Sahiwal cows in our studied population. The least-squares analysis showed a significant association (p < 0.05) of identified genetic variants with total milk yield (TMY) and 305 days milk yield (MY) (p < 0.05). The GG genotyped cows were found to be associated with higher milk yields. However, for reproduction traits under study AA genotype was found to be more favorable with respect to service period and calving interval (p < 0.05). Computational analysis was also performed to predict changes if any in the transcription factor binding sites (TFBS) caused due to the identified SNP in the intronic region of the leptin gene. TFBS analysis predicted that the targeted SNP at g.92450765G > A may lead to the disappearance of TFBS such as Hypermethylated in cancer 2 protein (HIC2), Max-binding protein MNT (MNT), Cyclic AMP-dependent transcription factor −3 (ATF3), Myc-associated factor X (MAX) and Microphthalmia-associated transcription factor (MITF). It may lead to changes in transcriptional regulation.

摘要

本研究对红牛瘦素基因候选单核苷酸多态性（SNP）g.92450765 G > A进行了探索，旨在探讨其对生产和繁殖性状的可能影响。BsaA 1 基因型 SNP g.92450765 G > A 的基因型和等位基因频率表明 AG (0.65) 基因型 Sahiwal 奶牛在我们的研究群体中占主导地位。最小二乘分析显示， 已识别的遗传变异与总产奶量 (TMY) 和 305 天产奶量 (MY) ( p < 0.05) 存在显着相关性 ( p  < 0.05)。研究发现，GG 基因分型的奶牛与较高的产奶量有关。然而，对于研究中的繁殖性状，发现 AA 基因型在服务期和产犊间隔方面更有利（p  < 0.05）。还进行了计算分析，以预测由于瘦素基因内含子区域中已识别的 SNP 引起的转录因子结合位点 (TFBS) 中是否有任何变化。TFBS 分析预测 g.92450765G > A 处的靶向 SNP 可能导致 TFBS 的消失，例如癌症 2 蛋白中的高甲基化 (HIC2)、最大结合蛋白 MNT (MNT)、环 AMP 依赖性转录因子 -3 (ATF3) ）、Myc 相关因子 X (MAX) 和小眼相关转录因子 (MITF)。它可能导致转录调控的变化。