Abstract

In this study, the effect of genetic variations of four heat shock transcription factor genes (HSF1, HSF2, HSF4, and HSF5) on the 3 D protein structure and function were studied. We defined the breed-specific genetic variations of pooled DNA of Tali goat that differed from the goat reference sequence (CHI2.0). Disordered regions of HSF proteins were predicted using PONDR. Post-translation changes were studied by several predicted online servers. Then, the structure of the order region of proteins was anticipated by using the Swiss model. Tali goat HSF genes contain a total number of 181, 679, 91, and 301 SNPs for HSF1, 2, 4, and 5, respectively. Also, 5 and 3 variants were identified as nsSNPs in the coding region of HSF4 and HSF5, respectively. (r.145A/S), (r.322P/Y), (r.379T/C) in HSF4 and (r.300Q/P), (r.573E/Q) in HSF5 obtained the tolerant and high confidence (SIFT score) for nsSNPs. More than half of these proteins are predicted to be disordered (56, 50, 52, and 50%, respectively for HSF1, 2, 4, and 5). Phosphorylation, acetylation, glycosylation, and Sumoylation sites of HSFs were compared between Tali goat and reference goat. Three residues S145, S263, and S322 of HSF4 in Tali goat were phosphorylation sites, and in HSF5, the reference goat has a phosphorylation site in S593.

摘要

在这项研究中，研究了四种热休克转录因子基因（HSF1、HSF2、HSF4 和 HSF5）的遗传变异对 3D 蛋白质结构和功能的影响。我们定义了与山羊参考序列 (CHI2.0) 不同的大里山羊混合 DNA 的品种特异性遗传变异。使用 PONDR 预测 HSF 蛋白的无序区域。几个预测的在线服务器研究了翻译后的变化。然后，使用瑞士模型预测蛋白质有序区域的结构。大里山羊 HSF 基因分别包含 181、679、91 和 301 个 SNP，分别对应 HSF1、2、4 和 5。此外，HSF4 和 HSF5 的编码区分别有 5 个和 3 个变体被鉴定为 nsSNP。(r.145A/S), (r.322P/Y), (r.379T/C) 在 HSF4 和 (r.300Q/P), (r. HSF5 中的 573E/Q) 获得了 nsSNP 的耐受性和高置信度（SIFT 分数）。预计这些蛋白质中有一半以上是无序的（HSF1、2、4 和 5 分别为 56%、50%、52% 和 50%）。在大里山羊和参考山羊之间比较了 HSF 的磷酸化、乙酰化、糖基化和苏木化位点。塔里山羊HSF4的三个残基S145、S263和S322是磷酸化位点，而在HSF5中，参考山羊在S593有一个磷酸化位点。