Glioma is the most common malignancy of the nervous system with high rates of recurrence and mortality, even after surgery. The 5-year survival rate is only about 5%. NEK8 is involved in multiple biological processes in a variety of cancers; however, its role in glioma is still not clear. In the current study, we evaluated the prognostic value of NEK8, as well as its role in the pathogenesis of glioma. Using a bioinformatics approach and RNA-seq data from public databases, we found that NEK8 expression is elevated in glioma tissues; we further verified this result by RT-PCR, Western blotting and immunochemistry using clinical samples. Functional enrichment analyses of genes with correlated expression indicated that elevated NEK8 expression is associated with increased immune cell infiltration in glioma and may affect the tumour microenvironment via the regulation of DNA damage/repair. Survival analyses revealed that high levels of NEK8 are associated with a poorer prognosis; higher WHO grade, IDH status, 1p/19q codeletion, age and NEK8 were identified as an independent prognostic factor. These findings support the crucial role of NEK8 in the progression of glioma via effects on immune cell infiltration and suggest that it is a new prognostic biomarker.

中文翻译：

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NEK8预后价值的生物信息学分析及其对胶质瘤免疫细胞浸润的影响

胶质瘤是最常见的神经系统恶性肿瘤，即使在手术后也具有很高的复发率和死亡率。5年生存率只有5%左右。NEK8 参与多种癌症的多种生物学过程；然而，它在胶质瘤中的作用仍不清楚。在目前的研究中，我们评估了 NEK8 的预后价值，以及它在胶质瘤发病机制中的作用。使用来自公共数据库的生物信息学方法和 RNA-seq 数据，我们发现神经胶质瘤组织中 NEK8 的表达升高。我们使用临床样本通过 RT-PCR、蛋白质印迹和免疫化学进一步验证了这一结果。具有相关表达的基因的功能富集分析表明，升高的 NEK8 表达与胶质瘤中免疫细胞浸润的增加有关，并且可能通过调节 DNA 损伤/修复来影响肿瘤微环境。生存分析表明，高水平的 NEK8 与较差的预后相关。较高的 WHO 分级、IDH 状态、1p/19q 共缺失、年龄和 NEK8 被确定为独立的预后因素。这些发现支持 NEK8 通过对免疫细胞浸润的影响在胶质瘤进展中的关键作用，并表明它是一种新的预后生物标志物。年龄和 NEK8 被确定为独立的预后因素。这些发现支持 NEK8 通过对免疫细胞浸润的影响在胶质瘤进展中的关键作用，并表明它是一种新的预后生物标志物。年龄和 NEK8 被确定为独立的预后因素。这些发现支持 NEK8 通过对免疫细胞浸润的影响在胶质瘤进展中的关键作用，并表明它是一种新的预后生物标志物。