Mastering the SNP content in the HLA region can be based on it to judiciously select unrelated donor stem cells with preferable MHC matching to lower postoperative complications. Herein, quantitative PCR-based primers and probes were designed for 10 transplants outcome-associated SNP loci with two-allelic polymorphism, and then a new detection system (“HLA-10-SNP”) was established. Compared with Sanger sequencing, its accuracy has been proven to reach 100%. Additionally, fluorescent PCR typing of 10 important SNPs via this system expressed excellent repeatability (sensitivity, 20 ng). Overall, the new system achieves single-sample classification precision and easily distinguishable results, equipped with the advantages of simple, rapid, accurate, and effective, promising to acquire widespread popularization and application in clinical settings.

中文翻译：

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“HLA-10-SNP”：一种新的基于 qPCR 的系统，可快速、有效和准确地检测 HLA 区域的 10 个重要 SNP

掌握HLA区域的SNP含量，可以以此为基础，明智地选择MHC匹配较好的无关供体干细胞，降低术后并发症。在此，针对具有双等位基因多态性的 10 个移植结果相关 SNP 位点设计了基于定量 PCR 的引物和探针，然后建立了一个新的检测系统（“HLA-10-SNP”）。与Sanger测序相比，其准确率已被证明达到100%。此外，通过该系统对 10 个重要 SNP 进行荧光 PCR 分型表现出出色的重复性（灵敏度，20 ng）。总体而言，新系统实现了单样本分类精度高、结果易于区分，具有简便、快速、准确、有效的优点，有望在临床上得到广泛的推广应用。