Long noncoding RNA (lncRNA) CDKN2B‑antisense RNA 1 (AS1) functions as a tumor oncogene in numerous cancers. However, the roles and mechanism of CDKN2B‑AS1 in colorectal cancer (CRC) have not been explored. The present study aimed to investigate whether and how CDKN2B‑AS1 contributes to CRC progression. The data revealed that CDKN2B‑AS1 expression was upregulated in CRC tissues. Loss‑of‑function assays demonstrated that CDKN2B‑AS1 in CRC modulated cell proliferation and apoptosis, which was mediated by cyclin D1, cyclin‑dependent kinase (CDK) 4, p‑Rb, caspase‑9 and caspase‑3. Bioinformatics analysis and luciferase reporter assays indicated direct binding of microRNA (miR)‑28‑5p to CDKN2B‑AS1. Moreover, the results herein revealed that the expression of miR‑28‑5p was negatively correlated with that of CDKN2B‑AS1 in CRC tissue. Moreover, CDKN2B‑AS1 acted as a miR‑28‑5p competing endogenous RNA (ceRNA) to target and regulate the expression of URGCP. These findings indicated that CDKN2B‑AS1 plays roles in CRC progression, providing a potential therapeutic target or novel diagnostic biomarker for CRC.

中文翻译：

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LncRNA CDKN2B-AS1 海绵 miR-28-5p 以调节结肠直肠癌的增殖和抑制细胞凋亡。

长链非编码 RNA (lncRNA) CDKN2B 反义 RNA 1 (AS1) 在多种癌症中作为肿瘤癌基因发挥作用。然而，CDKN2B-AS1 在结直肠癌 (CRC) 中的作用和机制尚未得到探索。本研究旨在调查 CDKN2B-AS1 是否以及如何促进 CRC 进展。数据显示 CDKN2B-AS1 表达在 CRC 组织中上调。功能丧失分析表明，CRC 中的 CDKN2B-AS1 调节细胞增殖和凋亡，这由细胞周期蛋白 D1、细胞周期蛋白依赖性激酶 (CDK) 4、p-Rb、caspase-9 和 caspase-3 介导。生物信息学分析和荧光素酶报告基因检测表明 microRNA (miR)-28-5p 与 CDKN2B-AS1 直接结合。此外，本文的结果表明，miR-28-5p 的表达与 CRC 组织中的 CDKN2B-AS1 的表达呈负相关。而且，CDKN2B-AS1 作为 miR-28-5p 竞争性内源 RNA (ceRNA) 靶向并调节 URGCP 的表达。这些发现表明 CDKN2B-AS1 在 CRC 进展中发挥作用，为 CRC 提供了潜在的治疗靶点或新的诊断生物标志物。