Rice kernel smut, caused by Tilletia barclayana, is an important fungal disease in rice worldwide. It can cause considerable losses of yield and milling quality in epidemic years. Having a culture medium able to stimulate the sporulation of the fungus is desirable for in vitro, greenhouse and field studies. However, no research has been conducted to develop optimal culture media for the growth and sporulation of T. barclayana. In this research, studies were conducted using four isolates of the fungus to evaluate various agar media, carbon sources, and incubation periods on the growth and sporulation of this fungus. Of the six culture media evaluated, rice bran agar (RBA) was the best medium in promoting sporulation, producing secondary sporidia of up to 1.95 × 10⁶ sporidia/ml, while the water agar-only medium produced the least number of secondary sporidia (< 2 × 10⁵ sporidia/ml). Potato dextrose agar (PDA) and potato sucrose agar (PSA) were the two media most effective in stimulating mycelial growth. Carbon source (sucrose, dextrose, and D-sorbitol) in culture media significantly affected the sporulation of the fungus but not mycelium growth. Incubation at 28 °C for 2 weeks under a 12 h light – 12 h darkness cycle resulted in the greatest sporulation of the four incubation periods (1, 2, 3 and 4 weeks) evaluated. The best culture medium and incubation time identified from this study could help facilitate the preparation of conidial inoculum for in vitro, greenhouse and field studies of kernel smut in rice.

稻米黑穗病是由黑粉虱引起的一种重要的水稻真菌病害。在流行年份，它会导致产量和碾磨质量的相当大损失。具有能够刺激真菌孢子形成的培养基对于体外、温室和田间研究是可取的。然而，尚未进行研究以开发用于T. barclayana生长和孢子形成的最佳培养基。在这项研究中，使用四种真菌分离物进行了研究，以评估各种琼脂培养基、碳源和潜伏期对这种真菌的生长和孢子形成的影响。在评估的六种培养基中，米糠琼脂 (RBA) 是促进孢子形成的最佳培养基，可产生高达 1.95 × 10 ^{6 的}次生孢子sporidia/ml，而纯水琼脂培养基产生最少数量的次生孢子 (< 2 × 10 ⁵孢子/ml)。马铃薯葡萄糖琼脂 (PDA) 和马铃薯蔗糖琼脂 (PSA) 是刺激菌丝生长最有效的两种培养基。培养基中的碳源（蔗糖、葡萄糖和 D-山梨糖醇）显着影响真菌的孢子形成，但不影响菌丝体的生长。在 12 小时光照 - 12 小时黑暗循环下，在 28 °C 下孵育 2 周，在所评估的四个孵育期（1、2、3 和 4 周）中产生最大的孢子形成。从这项研究中确定的最佳培养基和孵化时间有助于促进分生孢子接种物的制备，用于水稻籽粒黑穗病的体外、温室和田间研究。