To elucidate the pathogenesis of prostate diseases, following in silico analysis, the LKB1 gene was selected for further investigation. The LKB1 gene has been associated with poor prognosis and is frequently mutated in different types of cancers. In this study, 50 benign prostatic hyperplasia (BPH) and 57 prostate cancer (PCa) tissues, including matched normal tissue for the patients, were analyzed by qRT-PCR and DNA sequencing for LKB1 expression and the mutation profile, respectively. Expression of LKB1 was increased in 60.7% of the PCa tissues compared with noncancerous tissue samples (p ≤ 0.001). However, LKB1 expression was lower when compared with normal tissues in BPH (p = 0.920). Four coding sequence alterations were detected in BPH. Three silent mutations were located in codons 9, 32, and 275 and a missense mutation was observed in codon 384. Six alterations were identified in the intronic regions of the LKB1 gene in both PCa and BPH. Five mutations were observed in both patient groups. A new alteration in intron 6 was observed in a patient with PCa. The LKB1 gene may be associated with benign transformations rather than the tumors in prostate pathogenesis when its expression and mutation status are considered. However, the mechanism of LKB1 in PCa needs further studies.

中文翻译：

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LKB1 在前列腺癌中没有抑癌作用

为了阐明前列腺疾病的发病机制，在计算机分析之后，选择了LKB1基因进行进一步研究。在LKB1基因已经与不良预后以及在不同类型的癌症中常见的突变。在这项研究中，50良性前列腺增生（BPH）和前列腺57癌（PCa）组织，包括对患者匹配的正常组织，通过qRT-PCR和DNA测序所分析LKB1分别表达和突变谱。与非癌组织样本相比，LKB1 的表达在 60.7% 的 PCa 组织中增加（p  ≤ 0.001）。然而，LKB1与 BPH 中的正常组织相比，表达较低 ( p  = 0.920)。在 BPH 中检测到四种编码序列改变。三个沉默突变位于密码子 9、32 和 275，在密码子 384 中观察到错义突变。在 PCa 和 BPH的LKB1基因的内含子区域中发现了六个改变。在两个患者组中均观察到五个突变。在 PCa 患者中观察到内含子 6 的新变化。考虑到LKB1基因的表达和突变状态，它可能与良性转化有关，而不是与前列腺发病机制中的肿瘤有关。然而，LKB1在 PCa 中的作用机制需要进一步研究。