The purpose of the study was to investigate the effect of clinoptilolite zeolite and vermiculite on (i) nitrogen (N) and phosphorous (P) acquiring enzymes’ activity, (ii) potential nitrification rate (PNR), and on (iii) availabilities of the inorganic N forms. These parameters are critical to description of N mineralization and nitrification processes regulating the fate of N in agricultural ecosystems. A time-course (60-days) microcosm study was conducted on a circumneutral agricultural soil, where clinoptilolite zeolite and vermiculite were added as a mixture with N substrate (i.e., urea or manure). Zeolite inhibited urease activity regardless of the N supply (manure or urea). On the contrary, the activities of acid phosphatase, alkaline phosphatase, N-acetylglucosaminidase (NAG), asparaginase, glutaminase, and arylamidase remained unaffected. Moreover, zeolite and vermiculite affected the NH₄⁺ availability and retarded the NO₃⁻ accumulation in the soil, by 22 and 25%, respectively. Zeolite and vermiculite amendments showed lower potential nitrification rate (PNR) than single urea ones, by 58 and 45%, respectively, at day 15. Clinoptilolite zeolite and vermiculite affected nitrification activity and potentials of soil under organic and inorganic N amendment, whereas zeolite inhibited urease activity, suggesting an influence on the overall biochemical cycle of N. Further work is needed to elucidate as follows: (i) the responsible mechanism(s) behind the inhibited urease activity, (ii) the response of decomposing and nitrifying microorganisms, and (iii) the implications on N transformation rates.

本研究的目的是研究斜发沸石和蛭石对 (i) 氮 (N) 和磷 (P) 获得酶活性、(ii) 潜在硝化率 (PNR) 和 (iii) 可利用性的影响无机氮形式。这些参数对于描述调节农业生态系统中氮归宿的氮矿化和硝化过程至关重要。在中性农业土壤周围进行了时间进程（60 天）微观研究，其中斜发沸石沸石和蛭石作为与 N 底物（即尿素或粪肥）的混合物添加。无论氮供应（粪便或尿素）如何，沸石都会抑制脲酶活性。反之，酸性磷酸酶、碱性磷酸酶、N-乙酰氨基葡萄糖苷酶 (NAG)、天冬酰胺酶、谷氨酰胺酶和芳基酰胺酶不受影响。此外，沸石和蛭石影响了 NH ₄ ^{+ 的}可用性并延迟了 NO ₃ ^-在土壤中的积累，分别为 22% 和 25%。在第 15 天，沸石和蛭石添加物的潜在硝化率 (PNR) 比单一尿素添加物低 58% 和 45%。斜发沸石沸石和蛭石影响有机和无机氮添加物下土壤的硝化活性和潜力，而沸石抑制尿素酶活性，表明对 N 的整体生化循环有影响。 需要进一步的工作来阐明如下：（i）尿素酶活性受抑制背后的负责机制，（ii）分解和硝化微生物的反应，以及(iii) 对氮转化率的影响。