The efforts of the Global Poliovirus Eradication Initiative (GPEI) have brought about the near elimination of poliovirus worldwide. The World Health Organization has issued guidelines for the safe handling and containment of infectious materials (IM) and potentially infectious materials (PIM) following poliovirus eradication. Inactivation of poliovirus in IM and PIM is needed to prevent inadvertent re-introduction of polioviruses post-eradication. In this study, we investigated the use of guanidine thiocyanate-based nucleic acid extraction buffers from commercially available nucleic acid extraction kits to inactivate poliovirus in cell culture isolates and stool suspensions, two common types of poliovirus IM and PIM, respectively. Incubation with selected nucleic acid extraction buffers or extraction buffers supplemented with ethanol reduced the infectivity of high-titer wild poliovirus type 1 (WPV1), wild poliovirus type 3 (WPV3), Sabin 1 (SL1), and Sabin 3 (SL3) cell culture isolates below the limit of detection in CCID₅₀ assays. Stool suspensions containing WPV1, WPV3, SL1, SL2, or SL3 were also inactivated by the extraction buffers tested. Blind passage of WPV1-spiked stool suspensions confirmed complete inactivation of WPV1 after incubation with extraction buffers. Moreover, treatment with a buffer consisting of 4 M guanidine thiocyanate with 30 % ethanol inactivated a high-titer WPV1 culture isolate and a WPV1-spiked stool suspension. Taken together, these results show that guanidine thiocyanate-based nucleic acid extraction buffers are an effective means of inactivating poliovirus IM and PIM, and thus will be instrumental in ensuring containment compliance and preventing potential re-emergence of contained polioviruses.

全球消灭脊灰病毒倡议 (GPEI) 的努力已在全球范围内实现了几乎消灭脊灰病毒。世界卫生组织已经发布了脊髓灰质炎病毒根除后感染材料 (IM) 和潜在感染材料 (PIM) 的安全处理和控制指南。需要在 IM 和 PIM 中灭活脊髓灰质炎病毒，以防止在根除后无意中重新引入脊髓灰质炎病毒。在这项研究中，我们研究了使用市售核酸提取试剂盒中基于硫氰酸胍的核酸提取缓冲液来灭活细胞培养分离物和粪便悬浮液中的脊髓灰质炎病毒，这两种常见类型的脊髓灰质炎病毒 IM 和 PIM 分别是。₅₀次检测。含有 WPV1、WPV3、SL1、SL2 或 SL3 的粪便悬浮液也被测试的提取缓冲液灭活。WPV1 加标粪便悬浮液的盲通过证实了 WPV1 在与提取缓冲液孵育后完全失活。此外，使用由 4 M 硫氰酸胍和 30% 乙醇组成的缓冲液处理，可灭活高效价 WPV1 培养分离物和加标 WPV1 的粪便悬浮液。总之，这些结果表明，基于硫氰酸胍的核酸提取缓冲液是灭活脊髓灰质炎病毒 IM 和 PIM 的有效手段，因此将有助于确保遏制合规性并防止所含脊髓灰质炎病毒可能再次出现。