Monatshefte für Chemie - Chemical Monthly ( IF 1.7 ) Pub Date : 2021-08-07 , DOI: 10.1007/s00706-021-02816-5 Sanjeev Kumar Sharma 1 , William Fraser 1
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Fluorophore 1,8-naphthilamide was linked to 2-bromoacridine through an ethylenediamine spacer using a succinct synthetic route to give a bromoacridine-linked, bifunctional fluorophore conjugate for the detection of triplex DNA. Acridine is well known to intercalate into duplex DNA whereas introduction of a bulky bromine atom at position C2 redirects specificity for triplex over duplex DNA. In this work, photoelectron transfer assay was used to demonstrate that the synthesised 2-bromoacridine-linked fluorophore conjugate had good selectivity for the representative triplex DNA target sequence d(T*A.T)20 compared with double-stranded d(T.A)20, single-stranded dT20 or d(G/A)19 DNA sequences.
Graphic abstract
中文翻译：
含溴吖啶的荧光团对三链 DNA 的选择性
使用简洁的合成路线，荧光团 1,8-萘甲酰胺通过乙二胺间隔基与 2-溴吖啶连接，得到溴吖啶连接的双功能荧光团偶联物，用于检测三链 DNA。众所周知，吖啶嵌入双链 DNA 中，而在 C2 位置引入庞大的溴原子会重定向双链 DNA 对三链的特异性。在这项工作中，光电子转移试验用于证明合成的 2-溴吖啶连接的荧光团缀合物对代表性的三链 DNA 靶序列 d(T*AT) 20与双链 d(TA) 20相比具有良好的选择性，单链-链 dT 20或 d(G/A) 19 DNA 序列。
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