This study aimed to evaluate the effects of omega-3 (ω3) polyunsaturated fatty acids, in association with aspirin (AA), on the morphology of cytokine release in the temporomandibular joint (TMJ) of rats induced with rheumatoid arthritis (IR) by injecting 100 μL of complete Freund’s adjuvant with bovine type II collagen at the tail base. Thirty-two adult male rats were divided into treatment groups: Sham, treated with 0.9% sodium chloride (NaCl) p.o.; IR-control, treated with 0.9% NaCl p.o.; IR-ω3 treated with ω3 PUFAS (85 mg/kg/day p.o.); and IR-ω3 + AA treated with ω3 (85 mg/kg/day p.o.) + AA (20 mg/kg/day i.p.). After maintained treatment for seven days, the animals were euthanized. Bilateral TMJs from each rat were removed and one was subjected to histological immunoassays and enzyme-linked immunosorbent assays to assess interleukin (IL)-1β, tumor necrosis factor-α, and IL-10 levels. Data analysis was performed using the Kruskal–Wallis and Dunn tests. In the IR-ω3 and IR-ω3 + AA groups, the TMJ was greater than in the IR-control group (P < 0.0001). The addition of AA did not improve the effects of ω3 (P = 0.0698). Similarly, the addition of AA conferred no additional effects on the cytokine levels (P > 0.05); however, it increased the proteoglycan density, compared with ω3 alone. We found that ω3 exhibited anti-inflammatory activity in arthritic rats, and the addition of AA increased proteoglycan density, but did not affect cytokine expression.

本研究旨在评估 omega-3 (ω3) 多不饱和脂肪酸与阿司匹林 (AA) 联合注射对类风湿关节炎 (IR) 大鼠颞下颌关节 (TMJ) 细胞因子释放形态的影响。 100 μL 完全弗氏佐剂，尾基处含有牛 II 型胶原蛋白。将 32 只成年雄性大鼠分为治疗组：Sham，用 0.9% 氯化钠 (NaCl) po 治疗；IR-控制，用 0.9% NaCl po 处理；IR-ω3 用 ω3 PUFAS (85 mg/kg/day po) 处理；和 IR-ω3 + AA 用 ω3 (85 mg/kg/day po) + AA (20 mg/kg/day ip) 处理。维持治疗7天后，将动物安乐死。去除每只大鼠的双侧 TMJ，对一只大鼠进行组织学免疫测定和酶联免疫吸附测定以评估白细胞介素 (IL)-1β，肿瘤坏死因子-α和IL-10水平。使用 Kruskal-Wallis 和 Dunn 检验进行数据分析。在 IR-ω3 和 IR-ω3 + AA 组中，TMJ 大于 IR 对照组（P < 0.0001)。添加 AA 并没有改善 ω3 的效果（P = 0.0698）。同样，添加 AA 对细胞因子水平没有额外影响（P > 0.05）；然而，与单独的 ω3 相比，它增加了蛋白多糖的密度。我们发现 ω3 在关节炎大鼠中表现出抗炎活性，并且添加 AA 增加了蛋白多糖的密度，但不影响细胞因子的表达。