Broad bean wilt virus 2 (BBWV2) is an evolutionarily successful RNA virus with an extensive host range and worldwide distribution that causes severe damage to crops. While numerous BBWV2 isolates from various plant species have been identified and their genome sequences determined, little information is available on the virulence and symptomatic characteristics corresponding to the genomic sequences. In this study, we provide integrated information on the molecular and pathogenic characteristics of three genetically distant BBWV2 isolates: BBWV2-PC, -LS2, and P3 obtained from Gentiana scabra, Leonurus sibiricus, and Pisum sativum, respectively. Phylogenetic and diversity analyses of the BBWV2 population included 42 isolates from various host species and revealed that RNA2 has higher genetic plasticity than RNA1 and may have evolved under host-imposed constraints. In addition, we generated an infectious cDNA clone of BBWV2-PC RNA2 (pBBWV2-PC-R2). Pseudo-recombination analysis of pBBWV2-PC-R2 further demonstrated that RNA2 determines the pathogenic characteristics of the PC isolate.

蚕豆枯萎病毒 2 (BBWV2) 是一种进化成功的 RNA 病毒，具有广泛的宿主范围和全球分布，对作物造成严重损害。虽然已经鉴定了来自各种植物物种的大量 BBWV2 分离物并确定了它们的基因组序列，但关于基因组序列对应的毒力和症状特征的信息很少。在这项研究中，我们提供了关于三个遗传距离较远的 BBWV2 分离株的分子和致病特征的综合信息：BBWV2-PC、-LS2 和 P3，它们来自龙胆、西伯利亚狮子和豌豆。， 分别。BBWV2 种群的系统发育和多样性分析包括来自不同宿主物种的 42 个分离株，并显示 RNA2 具有比 RNA1 更高的遗传可塑性，并且可能在宿主施加的限制下进化。此外，我们生成了 BBWV2-PC RNA2 (pBBWV2-PC-R2) 的感染性 cDNA 克隆。pBBWV2-PC-R2 的假重组分析进一步表明，RNA2 决定了 PC 分离物的致病特征。