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Validation of the Microreader 28A ID System: A 6-dye multiplex amplification assay for forensic application
Electrophoresis ( IF 3.0 ) Pub Date : 2021-08-09 , DOI: 10.1002/elps.202100110
Lu Yin 1 , Jing Zhu 2 , Shengqiu Qu 1 , Yifan Li 3 , Yuqing Liu 4 , Zailiang Yu 5 , Fan Yang 5 , Ranran Zhang 1 , Jiaming Xue 1 , Sicheng Huang 6 , Weibo Liang 1
Affiliation  

The Microreader 28A ID System is a new 28-plex genotyping system with 6-dye multiplex amplification, which allows the simultaneous amplification of all 20 Combined DNA Index System (CODIS) core loci (CSF1PO, D3S1358, D5S818, D7S820, D8S1179, D13S317, D16S539, D18S51, D21S11, FGA, TH01, TPOX, vWA, D1S1656, D2S441, D2S1338, D10S1248, D12S391, D19S433, D22S1045), plus five extended STRs loci (D6S1043, Penta D, Penta E, DYS391, SE33), 2 Y-Indels (Rs2032678, Rs771783753), and the amelogenin loci. This system can be used for forensic analyses, such as personal identification, kinship testing, scientific research, database applications, and other aspects of human genetic identification.

中文翻译:

Microreader 28A ID 系统的验证:用于法医应用的 6 染料多重扩增测定

Microreader 28A ID 系统是一种新的 28 重基因分型系统,具有 6 种染料多重扩增,可同时扩增所有 20 个组合 DNA 索引系统 (CODIS) 核心基因座(CSF1PO、D3S1358、D5S818、D7S820、D8S1179、D13S317、 D16S539、D18S51、D21S11、FGA、TH01、TPOX、vWA、D1S1656、D2S441、D2S1338、D10S1248、D12S391、D19S433、D22S1045),以及五个扩展的 STR 基因座(D6S1043、Penta D、Penta E、2DYS39) -Indels (Rs2032678、Rs771783753) 和牙釉蛋白基因座。该系统可用于法医分析,如个人识别、亲属关系检测、科学研究、数据库应用以及人类基因鉴定的其他方面。
更新日期:2021-10-06
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